TY - JOUR
T1 - Cytoplasmic progesterone receptors in the hypothalamus-preoptic area of the mouse
T2 - Effect of estrogen priming
AU - Roselli, Charles E.
AU - Snipes, Charles A.
N1 - Funding Information:
*Parts of this paper were presented as an abstract (No. 866) at the 63rd annual meeting of The Endocrine Society, Cincinnati, OH (1981). The work was supported in part by National Institutes of Health Biomedical Research Support Grant S-SO7 RR-05413. tPresent address: Reproductive Physiology. Oregon Regional Primate Research Center, 505 N.W. 185th Avenue, Beaverton, OR 97006. *This work partially fulfilled the requirements for a Ph.D. degree for CER from Hahnemann University, Philadelphia. PA. DAddress reprint requests to Charles A. Snipes, Ph.D.. Department of Physiology and Biophysics, Hahnemann University, Philadelphia, PA 19102. The abbreviations and trivial steroid nomenclature used in this paper are: B. percentage of radioligand bound in the presence of competitor: B,,, percentage of radioligand bound in the absence of competitor; B,, number of specific binding sites; cortisol, 1 I/j’,1 7,21-trihydroxy-4-pregnene-3,20-dione; 5a-dihydroprogesterone (Su-DHP), 5u-pregnane-3.20-dione; EDTA. ethylenediaminetetraacetic acid; HPOA. hypothalamus-preoptic area: K,,, apparent dissociation constant; 3a-0 1, 3a-hydroxy-5a-pregnan-20-one; progesterone, 4-pregnene-3,20-dione; R5020, 17a.2 l-dimethyl-19-norpregna-4.9-diene-3.20-dione; RBA, relative binding affinity; TED buffer, buffer comprising tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetic acid, and dithiothreitol; Tris, tris(hydroxymethyl)aminomethane.
PY - 1983/11
Y1 - 1983/11
N2 - A synthetic progestin, R5020, was used to identify cytoplasmic progestin receptors in the hypothalamuspreoptic area (HPOA) of ovariectomized mice. These high-affinity receptors exhibited an apparent dissociation constant of approx. 1 nM. The receptors were specific for progestins. [3H]R5020 binding was inhibited by more than 50% with a 50-fold excess of either radioinert R5020 or progesterone. 5α-Dihydroprogesterone inhibited binding to a lesser extent. 3α-Hydroxy-5α-pregnane 20-one and cortisol did not compete for [3H]R5020 binding. Administration of estradiol benzoate (10 μg), 48 h prior to death, resulted in a 54% increase in the HPOA progestin receptor concentration when compared to oil-injected controls. These data demonstrate that there are specific and saturable cytoplasmic progestin receptors in the mouse HPOA and that the concentration of these receptors is increased after estrogen treatment.
AB - A synthetic progestin, R5020, was used to identify cytoplasmic progestin receptors in the hypothalamuspreoptic area (HPOA) of ovariectomized mice. These high-affinity receptors exhibited an apparent dissociation constant of approx. 1 nM. The receptors were specific for progestins. [3H]R5020 binding was inhibited by more than 50% with a 50-fold excess of either radioinert R5020 or progesterone. 5α-Dihydroprogesterone inhibited binding to a lesser extent. 3α-Hydroxy-5α-pregnane 20-one and cortisol did not compete for [3H]R5020 binding. Administration of estradiol benzoate (10 μg), 48 h prior to death, resulted in a 54% increase in the HPOA progestin receptor concentration when compared to oil-injected controls. These data demonstrate that there are specific and saturable cytoplasmic progestin receptors in the mouse HPOA and that the concentration of these receptors is increased after estrogen treatment.
UR - http://www.scopus.com/inward/record.url?scp=0021063095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021063095&partnerID=8YFLogxK
U2 - 10.1016/0022-4731(83)90372-2
DO - 10.1016/0022-4731(83)90372-2
M3 - Article
C2 - 6685796
AN - SCOPUS:0021063095
SN - 0960-0760
VL - 19
SP - 1571
EP - 1575
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
IS - 5
ER -