Cytoplasmic progesterone receptors in the hypothalamus-preoptic area of the mouse

Effect of estrogen priming

Charles Roselli, Charles A. Snipes

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A synthetic progestin, R5020, was used to identify cytoplasmic progestin receptors in the hypothalamuspreoptic area (HPOA) of ovariectomized mice. These high-affinity receptors exhibited an apparent dissociation constant of approx. 1 nM. The receptors were specific for progestins. [3H]R5020 binding was inhibited by more than 50% with a 50-fold excess of either radioinert R5020 or progesterone. 5α-Dihydroprogesterone inhibited binding to a lesser extent. 3α-Hydroxy-5α-pregnane 20-one and cortisol did not compete for [3H]R5020 binding. Administration of estradiol benzoate (10 μg), 48 h prior to death, resulted in a 54% increase in the HPOA progestin receptor concentration when compared to oil-injected controls. These data demonstrate that there are specific and saturable cytoplasmic progestin receptors in the mouse HPOA and that the concentration of these receptors is increased after estrogen treatment.

Original languageEnglish (US)
Pages (from-to)1571-1575
Number of pages5
JournalJournal of Steroid Biochemistry
Volume19
Issue number5
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Promegestone
Preoptic Area
Progesterone Receptors
Cytoplasmic and Nuclear Receptors
Hypothalamus
Estrogens
20-alpha-Dihydroprogesterone
Progesterone Congeners
Progestins
Progesterone
Hydrocortisone
Oils

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Cytoplasmic progesterone receptors in the hypothalamus-preoptic area of the mouse : Effect of estrogen priming. / Roselli, Charles; Snipes, Charles A.

In: Journal of Steroid Biochemistry, Vol. 19, No. 5, 1983, p. 1571-1575.

Research output: Contribution to journalArticle

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abstract = "A synthetic progestin, R5020, was used to identify cytoplasmic progestin receptors in the hypothalamuspreoptic area (HPOA) of ovariectomized mice. These high-affinity receptors exhibited an apparent dissociation constant of approx. 1 nM. The receptors were specific for progestins. [3H]R5020 binding was inhibited by more than 50{\%} with a 50-fold excess of either radioinert R5020 or progesterone. 5α-Dihydroprogesterone inhibited binding to a lesser extent. 3α-Hydroxy-5α-pregnane 20-one and cortisol did not compete for [3H]R5020 binding. Administration of estradiol benzoate (10 μg), 48 h prior to death, resulted in a 54{\%} increase in the HPOA progestin receptor concentration when compared to oil-injected controls. These data demonstrate that there are specific and saturable cytoplasmic progestin receptors in the mouse HPOA and that the concentration of these receptors is increased after estrogen treatment.",
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