Cytomegalovirus latency promotes cardiac lymphoid neogenesis and accelerated allograft rejection in CMV Naïve recipients

Susan Orloff, Y. K. Hwee, C. Kreklywich, T. F. Andoh, E. Hart, P. A. Smith, I. Messaoudi, Daniel Streblow

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Human cytomegalovirus (HCMV) infection is associated with the acceleration of transplant vascular sclerosis (TVS) and chronic allograft rejection (CR). HCMV-negative recipients of latently HCMV infected donor grafts are at highest risk for developing CMV disease. Using a rat heart transplant CR model, we have previously shown that acute rat CMV (RCMV) infection following transplantation significantly accelerates both TVS and CR. Here, we report that RCMV-naïve recipients of heart allografts from latently RCMV-infected donors undergo acceleration of CR with similar kinetics as acutely infected recipients. In contrast to acutely infected recipients, treatment of recipients of latently infected donor hearts with ganciclovir did not prevent CR or TVS. We observed the formation of tertiary lymphoid structures (TLOs) containing macrophages and T cells in latently infected hearts prior to transplantation but not in uninfected rats. Moreover, pathway analysis of gene expression data from allografts from latently infected donors indicated an early and sustained production of TLO-associated genes compared to allografts from uninfected donors. We conclude that RCMV-induced TLO formation and alteration of donor tissue T cell profiles prior to transplantation in part mediate the ganciclovir-insensitive rejection of latently infected donor allografts transplanted into naïve recipients by providing a scaffold for immune activation. Intra-cardiac lymphoid structures develop during rat cytomegalovirus (RCMV) latency, and transplantation of these latently infected hearts into RCMV naïve rats enhances recipient immune activation and significantly accelerates the rate of rejection.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalAmerican Journal of Transplantation
Volume11
Issue number1
DOIs
StatePublished - Jan 2011

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Cytomegalovirus
Allografts
Arteriosclerosis
Transplantation
Transplants
Ganciclovir
Muromegalovirus
T-Lymphocytes
Cytomegalovirus Infections
Graft Rejection
Macrophages
Tissue Donors
Gene Expression
Infection
Genes

Keywords

  • Chronic rejection
  • cytomegalovirus
  • latency
  • transplant vascular sclerosis

ASJC Scopus subject areas

  • Transplantation
  • Immunology and Allergy
  • Pharmacology (medical)

Cite this

Cytomegalovirus latency promotes cardiac lymphoid neogenesis and accelerated allograft rejection in CMV Naïve recipients. / Orloff, Susan; Hwee, Y. K.; Kreklywich, C.; Andoh, T. F.; Hart, E.; Smith, P. A.; Messaoudi, I.; Streblow, Daniel.

In: American Journal of Transplantation, Vol. 11, No. 1, 01.2011, p. 45-55.

Research output: Contribution to journalArticle

Orloff, Susan ; Hwee, Y. K. ; Kreklywich, C. ; Andoh, T. F. ; Hart, E. ; Smith, P. A. ; Messaoudi, I. ; Streblow, Daniel. / Cytomegalovirus latency promotes cardiac lymphoid neogenesis and accelerated allograft rejection in CMV Naïve recipients. In: American Journal of Transplantation. 2011 ; Vol. 11, No. 1. pp. 45-55.
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