Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals

Florian Kern, Torsten Bunde, Nicole Faulhaber, Felix Kiecker, Elham Khatamzas, Ina Maria Rudawski, Axel Pruss, Jan Willem Gratama, Rudolf Volkmer-Engert, Ralf Ewert, Petra Reinke, Hans Dieter Volk, Louis J. Picker

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207 Scopus citations

Abstract

Antigen-specific, cytokine flow cytometry was used to analyze the prevalence and frequency of CD4 and CD8 memory T cells specific for the abundantly expressed cytomegalovirus (CMV) phosphoprotein 65 (pp65) in healthy CMV IgG-seropositive individuals. Stimulation of peripheral blood mononuclear cells with peptide pools and individual peptides derived from the pp65 amino acid sequence in 40 donors revealed that 63% of donors had a detectable CD4 T cell response and that 83% of donors had a detectable CD8 T cell response against this protein. The overall frequencies of T cells directed against pp65 were analyzed for 20 donors by stimulation with peptide pools covering the complete pp65 protein and were as high as 2 in 1000 and 9 in 1000 (median) peripheral blood CD4 and CD8 T cells, respectively. In addition, a comparison between CD4 responses to a CMV lysate containing various CMV proteins and pp65-specific responses in 9 donors indicated that pp65 was a dominant target of the CMV-specific CD4 T cell response in some, but not all, donors. Several new T cell epitopes were identified.

Original languageEnglish (US)
Pages (from-to)1709-1716
Number of pages8
JournalJournal of Infectious Diseases
Volume185
Issue number12
DOIs
StatePublished - Jun 15 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Kern, F., Bunde, T., Faulhaber, N., Kiecker, F., Khatamzas, E., Rudawski, I. M., Pruss, A., Gratama, J. W., Volkmer-Engert, R., Ewert, R., Reinke, P., Volk, H. D., & Picker, L. J. (2002). Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals. Journal of Infectious Diseases, 185(12), 1709-1716. https://doi.org/10.1086/340637