Cytokine response gene 8 (CR8) regulates the cell cycle G1-S phase transition and promotes cellular survival

Carol Beadling, Anna Cereseto, Wen Fan, Mayumi Naramura, Kendall A. Smith

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cellular proliferation and survival are modulated by the expression of specific genes. Cytokine response gene 8 (CR8), which was originally cloned as an IL-2-induced gene in human T lymphocytes, encodes a basic helix-loop-helix (bHLH) transcription factor. The CR8 gene product is highly conserved among human, mouse and rat, and contains sequence motifs that distinguish it from other bHLH families. The CR8 gene is ubiquitously expressed, and CR8 gene expression is induced by both growth-promoting as well as growth-inhibitory stimuli. As bHLH proteins have been found to regulate both the G1-S phase cell cycle transition, as well as cellular survival, the effects of CR8 on these processes were investigated. Ectopic CR8 expression in asynchronous U2OS cell cultures reduces the percentage of cells in the cell cycle S phase, and also slows the entry of G1-synchronized cells into S phase. The prolonged G1 interval correlates with impaired elevation of cyclin E protein and prolonged p21 protein expression in G1. CR8 expression also protects U2OS cells from serum-withdrawal induced apoptosis. These results indicate that CR8 is an important modulator of both the G1-S phase cell cycle transition, and cellular survival.

Original languageEnglish (US)
Pages (from-to)1771-1783
Number of pages13
JournalOncogene
Volume20
Issue number14
DOIs
StatePublished - Mar 29 2001
Externally publishedYes

Fingerprint

Phase Transition
G1 Phase
S Phase
Cell Cycle
Cytokines
Survival
Genes
Gene Expression
Basic Helix-Loop-Helix Transcription Factors
Cyclin E
Proteins
Growth
Interleukin-2
Cell Culture Techniques
Cell Proliferation
Apoptosis
T-Lymphocytes

Keywords

  • Basic helix-loop-helix
  • G1 progression
  • Survival

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Cytokine response gene 8 (CR8) regulates the cell cycle G1-S phase transition and promotes cellular survival. / Beadling, Carol; Cereseto, Anna; Fan, Wen; Naramura, Mayumi; Smith, Kendall A.

In: Oncogene, Vol. 20, No. 14, 29.03.2001, p. 1771-1783.

Research output: Contribution to journalArticle

Beadling, Carol ; Cereseto, Anna ; Fan, Wen ; Naramura, Mayumi ; Smith, Kendall A. / Cytokine response gene 8 (CR8) regulates the cell cycle G1-S phase transition and promotes cellular survival. In: Oncogene. 2001 ; Vol. 20, No. 14. pp. 1771-1783.
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