Cytochemical studies of metaphase chromosomes by flow cytometry

R. G. Langlois, A. V. Carrano, Joe Gray, M. A. Van Dilla

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The cytochemical properties of metaphase chromosomes from Chinese hamster and human cells were studied by flow cytometry. This technique allows precise quantitation of the fluorescence properties of individual stained chromosome types. Chromosomes were stained with the following fluorescent DNA stains: Hoechst 33258, DAPI, chromomycin A3, ethidium bromide, and propidium iodide. The relative fluorescence of individual chromosome types varied depending on the stain used, demonstrating that individual chromosome types differ in chemical properties. Flow measurements were performed as a function of stain and chromosome concentration to characterize the number and distribution of stain binding sites. Flow analysis of double stained chromosomes show that bound stains interact by energy transfer with little or no binding competition. For most hamster chromosomes, there is a strong correlation between relative fluorescence and stain base preference suggesting that staining differences may be determined primarily by differences in average base composition. A few hamster chromosome types exhibit anomalous staining which suggests that some other property, such as repetitive DNA sequences, also may be an important determinant of chromosomal staining.

Original languageEnglish (US)
Pages (from-to)229-251
Number of pages23
JournalChromosoma
Volume77
Issue number3
DOIs
StatePublished - Mar 1980
Externally publishedYes

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Metaphase
Flow Cytometry
Chromosomes
Coloring Agents
Fluorescence
Staining and Labeling
Cricetinae
Chromomycin A3
Bisbenzimidazole
Ethidium
Propidium
Nucleic Acid Repetitive Sequences
Energy Transfer
Base Composition
Cricetulus
Binding Sites
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Langlois, R. G., Carrano, A. V., Gray, J., & Van Dilla, M. A. (1980). Cytochemical studies of metaphase chromosomes by flow cytometry. Chromosoma, 77(3), 229-251. https://doi.org/10.1007/BF00286050

Cytochemical studies of metaphase chromosomes by flow cytometry. / Langlois, R. G.; Carrano, A. V.; Gray, Joe; Van Dilla, M. A.

In: Chromosoma, Vol. 77, No. 3, 03.1980, p. 229-251.

Research output: Contribution to journalArticle

Langlois, RG, Carrano, AV, Gray, J & Van Dilla, MA 1980, 'Cytochemical studies of metaphase chromosomes by flow cytometry', Chromosoma, vol. 77, no. 3, pp. 229-251. https://doi.org/10.1007/BF00286050
Langlois, R. G. ; Carrano, A. V. ; Gray, Joe ; Van Dilla, M. A. / Cytochemical studies of metaphase chromosomes by flow cytometry. In: Chromosoma. 1980 ; Vol. 77, No. 3. pp. 229-251.
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