Cyclosporin induces renal proto-oncogene RNA message and increased transforming growth factor-β prior to renal fibrosis: Modification by calcium channel blockade in the salt replete rat

Subodh J. Saggi, Takeshi F. Andoh, Robert Safirstein, William M. Bennett

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background: Chronic cyclosporin (CsA) administration has been shown to result in the replacement of epithelial cells in the kidney with fibrous tissue. These changes are kidney-specific, as they do not occur in any other organ. Results: Cyclosporin exposure increases c-fos and c-jun mRNA in the rat kidney but not in the liver. Furthermore, chronic CsA exposure causes a further increase in c-fos and c-jun mRNA and increases the renal expression of transforming growth factor-β (TGF-β) mRNA. These changes precede the development of fibrosis. The combined insult of ischaemia and CsA resulted in synergistic increases in c-fos, suggesting that CsA recruited a pathway for c-fos activation different from ischaemia. The calcium channel blocker, verapamil, blocked CsA-induced expression of c-fos and c-jun mRNA, and reduced the amount of TGF-β expression. Conclusion: These data are consistent with the notion that CsA induces protooncogenes, which may be, at least partially, responsible for long-term CsA nephrotoxicity.

Original languageEnglish (US)
Pages (from-to)58-64
Number of pages7
JournalNephrology
Volume9
Issue number2
DOIs
StatePublished - Apr 2004
Externally publishedYes

Keywords

  • c-fos
  • c-jun
  • Cyclosporin
  • Fibrosis
  • Transforming growth factor-β
  • Verapamil

ASJC Scopus subject areas

  • Nephrology

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