Cyclosporin a: Effects on the secretory process and noradrenergic activity in the submandibular gland of the rat

Juan M. Giglio, Marcelo S. Vatta, Liliana G. Bianciotti, Alejandro Lomniczi, Juan C. Elverdin

    Research output: Contribution to journalArticle

    2 Scopus citations

    Abstract

    Objectives: The aim of the present work was to study the effect of long-term cyclosporine (CSA) administration on norepinephrine (NE) metabolism and adrenergic-evoked secretion in the rat submandibular gland (SMG). Methods: Dose-response curves to adrenergic agonists (methoxamine, isoproterenol, NE) were performed in control and CSA (10 and 30 mg/kg every 2 days for 1 month) treated rats after SMG duct cannulation. In SMG tissue neuronal NE uptake, release, synthesis and endogenous content were determined. In addition phosphoinositide intracellular signaling was also investigated. Results: CSA administration caused an increase in salivary secretion evoked by methoxamine (α-adrenergic agonist) and NE but failed to modify salivation evoked by β-adrenergic stimulation (isoproterenol). Long-term CSA administration decreased NE release and synthesis whereas it enhanced the amine uptake and phosphoinositide hydrolysis in the SMG. Conclusions: The administration of CSA for 30 days induced salivary gland sensitization likely mediated by diminished adrenergic input. Present results suggest that the decreased sympathetic activity evoked by long-term CSA administration in the rat SMG may lead to sensitization of the gland supported by increased phosphoinositide hydrolysis and enhanced adrenergic-evoked salivation.

    Original languageEnglish (US)
    Pages (from-to)170-176
    Number of pages7
    JournalNeuroImmunoModulation
    Volume9
    Issue number3
    DOIs
    StatePublished - Dec 1 2001

    Keywords

    • Adrenergic receptors
    • Calcineurin
    • Inositol phosphates
    • Salivary glands
    • Salivary secretion

    ASJC Scopus subject areas

    • Immunology
    • Endocrinology
    • Neurology
    • Endocrine and Autonomic Systems

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