Cyclodextrin enhances spermicidal effects of magainin-2-amide

C. Wojcik, W. Sawicki, P. Marianowski, M. Benchaib, J. C. Czyba, J. F. Guerin

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Magainins are antimicrobial peptides with known spermicidal activity. Their activity is inhibited by cholesterol present in eukaryotic cell membranes. Pretreatment of spermatozoa with methyl-β-cyclodextrin, which extracts cholesterol from cell membranes and induces capacitation, sensitizes them to magainin-2-amide as shown by a decrease in human sperm motility determined by computer-assisted sperm analysis and a concomitant decrease in sperm viability, as measured by MitoTracker® Red CMXRos labeling. Magainin-2-amide affects mainly the fast progressive spermatozoa inducing them directly into an immotile state, without an increase in motile non-progressive and slow progressive spermatozoa. We conclude that methyl-β-cyclodextrin highly potentiates the deleterious effect of magainin-2-amide on human spermatozoa. Most probably, this effect can be explained by cholesterol extraction from sperm cell membranes. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalContraception
Volume62
Issue number2
DOIs
StatePublished - 2000

Keywords

  • Cyclodextrin
  • Human spermatozoa
  • Magainin-2-amide
  • Sperm motility
  • Sperm viability

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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