Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps

D. Khvostenko, S. Salehi, S. E. Naleway, Thomas (Tom) Hilton, Jack Ferracane, J. C. Mitchell, J. J. Kruzic

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objectives Secondary caries is the most common reason for composite restoration replacement and usually forms between dentin and the filling. The objective of this study was to investigate the combined effect of cyclic loading and bacterial exposure on bacterial penetration into gaps at the interface between dentin and resin composite restorative material using a novel bioreactor system and test specimen design. Methods Human molars were machined into 3 mm thick disks with 2 mm deep × 5 mm diameter cavity preparations into which composite restorations were placed. A ∼15-30 μm (small) or ∼300 μm wide (large) marginal gap was introduced along half of the interface between the dentin and restoration. Streptococcus mutans UA 159 biofilms were grown on each sample prior to testing each in a bioreactor both with and without cyclic loading. Both groups of samples were tested for 2 weeks and post-test biofilm viability was confirmed with a live-dead assay. Samples were fixed, mounted and cross-sectioned to reveal the gaps and observe the depth of bacterial penetration. Results It was shown that for large gap samples the bacteria easily penetrated to the full depth of the gap independent of loading or non-loading conditions. The results for all cyclically loaded small gap samples show a consistently deep bacterial penetration down 100% of the gap while the average penetration depth was only 67% for the non-loaded samples with only two of six samples reaching 100%. Significance A new bioreactor was developed that allows combining cyclic mechanical loading and bacterial exposure of restored teeth for bacterial biofilm and demineralization studies. Cyclic loading was shown to aid bacterial penetration into narrow marginal gaps, which could ultimately promote secondary caries formation.

Original languageEnglish (US)
Pages (from-to)702-710
Number of pages9
JournalDental Materials
Volume31
Issue number6
DOIs
StatePublished - Jun 1 2015

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Biofilms
Bioreactors
Dentin
Restoration
Composite materials
Streptococcus mutans
Composite Resins
Assays
Bacteria
Tooth
Resins
Testing

Keywords

  • Biofilm
  • Cyclic loading
  • Marginal gap
  • Resin composite
  • Secondary caries
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)
  • Materials Science(all)
  • Mechanics of Materials

Cite this

Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps. / Khvostenko, D.; Salehi, S.; Naleway, S. E.; Hilton, Thomas (Tom); Ferracane, Jack; Mitchell, J. C.; Kruzic, J. J.

In: Dental Materials, Vol. 31, No. 6, 01.06.2015, p. 702-710.

Research output: Contribution to journalArticle

Khvostenko, D. ; Salehi, S. ; Naleway, S. E. ; Hilton, Thomas (Tom) ; Ferracane, Jack ; Mitchell, J. C. ; Kruzic, J. J. / Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps. In: Dental Materials. 2015 ; Vol. 31, No. 6. pp. 702-710.
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abstract = "Objectives Secondary caries is the most common reason for composite restoration replacement and usually forms between dentin and the filling. The objective of this study was to investigate the combined effect of cyclic loading and bacterial exposure on bacterial penetration into gaps at the interface between dentin and resin composite restorative material using a novel bioreactor system and test specimen design. Methods Human molars were machined into 3 mm thick disks with 2 mm deep × 5 mm diameter cavity preparations into which composite restorations were placed. A ∼15-30 μm (small) or ∼300 μm wide (large) marginal gap was introduced along half of the interface between the dentin and restoration. Streptococcus mutans UA 159 biofilms were grown on each sample prior to testing each in a bioreactor both with and without cyclic loading. Both groups of samples were tested for 2 weeks and post-test biofilm viability was confirmed with a live-dead assay. Samples were fixed, mounted and cross-sectioned to reveal the gaps and observe the depth of bacterial penetration. Results It was shown that for large gap samples the bacteria easily penetrated to the full depth of the gap independent of loading or non-loading conditions. The results for all cyclically loaded small gap samples show a consistently deep bacterial penetration down 100{\%} of the gap while the average penetration depth was only 67{\%} for the non-loaded samples with only two of six samples reaching 100{\%}. Significance A new bioreactor was developed that allows combining cyclic mechanical loading and bacterial exposure of restored teeth for bacterial biofilm and demineralization studies. Cyclic loading was shown to aid bacterial penetration into narrow marginal gaps, which could ultimately promote secondary caries formation.",
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