Cyclic AMP, a nonessential regulator of the cell cycle

P. Coffino, Joe Gray, G. M. Tomkins

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Flow microfluorimetric analysis has been carried out on populations of exponentially growing S49 mouse lymphoma cells treated with dibutyryl cyclic AMP. The drug produces a specific concentration dependent block in the G1 phase of the cell cycle while other phases of the cycle are not perceptibly altered. The cell cycle of a line of mutant cells lacking the cyclic AMP dependent protein kinase is not affected by the drug. Since these mutant cells have been shown to maintain a normal cell cycle, even in the presence of high levels of cyclic AMP, periodic fluctuations in the levels of the cyclic nucleotide cannot be required for or determine progression through the cell cycle.

Original languageEnglish (US)
Pages (from-to)878-882
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number3
StatePublished - 1975
Externally publishedYes

Fingerprint

Cyclic AMP
Cell Cycle
Bucladesine
Cyclic Nucleotides
G1 Phase
Cyclic AMP-Dependent Protein Kinases
Pharmaceutical Preparations
Lymphoma
Population

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cyclic AMP, a nonessential regulator of the cell cycle. / Coffino, P.; Gray, Joe; Tomkins, G. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, No. 3, 1975, p. 878-882.

Research output: Contribution to journalArticle

@article{9931dc05028747ed9afc2880f5e2e1f9,
title = "Cyclic AMP, a nonessential regulator of the cell cycle",
abstract = "Flow microfluorimetric analysis has been carried out on populations of exponentially growing S49 mouse lymphoma cells treated with dibutyryl cyclic AMP. The drug produces a specific concentration dependent block in the G1 phase of the cell cycle while other phases of the cycle are not perceptibly altered. The cell cycle of a line of mutant cells lacking the cyclic AMP dependent protein kinase is not affected by the drug. Since these mutant cells have been shown to maintain a normal cell cycle, even in the presence of high levels of cyclic AMP, periodic fluctuations in the levels of the cyclic nucleotide cannot be required for or determine progression through the cell cycle.",
author = "P. Coffino and Joe Gray and Tomkins, {G. M.}",
year = "1975",
language = "English (US)",
volume = "72",
pages = "878--882",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "3",

}

TY - JOUR

T1 - Cyclic AMP, a nonessential regulator of the cell cycle

AU - Coffino, P.

AU - Gray, Joe

AU - Tomkins, G. M.

PY - 1975

Y1 - 1975

N2 - Flow microfluorimetric analysis has been carried out on populations of exponentially growing S49 mouse lymphoma cells treated with dibutyryl cyclic AMP. The drug produces a specific concentration dependent block in the G1 phase of the cell cycle while other phases of the cycle are not perceptibly altered. The cell cycle of a line of mutant cells lacking the cyclic AMP dependent protein kinase is not affected by the drug. Since these mutant cells have been shown to maintain a normal cell cycle, even in the presence of high levels of cyclic AMP, periodic fluctuations in the levels of the cyclic nucleotide cannot be required for or determine progression through the cell cycle.

AB - Flow microfluorimetric analysis has been carried out on populations of exponentially growing S49 mouse lymphoma cells treated with dibutyryl cyclic AMP. The drug produces a specific concentration dependent block in the G1 phase of the cell cycle while other phases of the cycle are not perceptibly altered. The cell cycle of a line of mutant cells lacking the cyclic AMP dependent protein kinase is not affected by the drug. Since these mutant cells have been shown to maintain a normal cell cycle, even in the presence of high levels of cyclic AMP, periodic fluctuations in the levels of the cyclic nucleotide cannot be required for or determine progression through the cell cycle.

UR - http://www.scopus.com/inward/record.url?scp=0016684871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016684871&partnerID=8YFLogxK

M3 - Article

C2 - 165491

AN - SCOPUS:0016684871

VL - 72

SP - 878

EP - 882

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 3

ER -