Abstract
Cell cycle progression delays and subsequent growth kinetics of viable and nonviable Chinese hamster cells following acute (45.5°) hyperthermia were documented in an attempt to correlate these changes with the decay of thermal tolerance. Following heating for various lengths of time, cells exhibited a delay in subsequent division which was related to cell survival by a power function relationship. A cell was considered to be viable if it retained the ability to divide to form a colony of 50 or more cells. The components of the delay in cycle transit for viable cells heated in G1 for treatment of 20 min at 45.5° were approximately 28 hr in G1 and 20 hr in S and G2-M. This represents a 7-fold decrease in the rate progression through G1 and a 2-fold decrease through S and G2-M relative to control rates. The doubling times of viable cells, in subsequent generations, were significantly decreased to a rate 61% of that of control up to 120 h after heating. This reduction was in part due to lethal sectoring, i.e., a division which produces only one daughter that is capable of forming a viable subclone, within the viable progeny. Within a viable subclone, up to 30% of the cells that divided from 48 to 91 hr after a heat treatment of 20 min at 45.5° were found to be nonclonogenic. Following resumption of division, nonviable cells slowly lost their capacity for proliferation. Nearly all thermal tolerance development induced by a 20-min pretreatment occurred while the viable cells remained in G1. Subsequent progression into heat-sensitive S and G2-M phases modulated thermal tolerance only slightly. Finally, maximal loss of thermal tolerance was exhibited at the time corresponding to the resumption of viable cell division.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1802-1808 |
| Number of pages | 7 |
| Journal | Cancer Research |
| Volume | 44 |
| Issue number | 5 |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Cycle progression and division of viable and nonviable Chinese hamster ovary cells following acute hyperthermia and their relationship to thermal tolerance decay. / Rice, G. C.; Gray, J. W.; Gray, Joe.
In: Cancer Research, Vol. 44, No. 5, 1984, p. 1802-1808.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cycle progression and division of viable and nonviable Chinese hamster ovary cells following acute hyperthermia and their relationship to thermal tolerance decay
AU - Rice, G. C.
AU - Gray, J. W.
AU - Gray, Joe
PY - 1984
Y1 - 1984
N2 - Cell cycle progression delays and subsequent growth kinetics of viable and nonviable Chinese hamster cells following acute (45.5°) hyperthermia were documented in an attempt to correlate these changes with the decay of thermal tolerance. Following heating for various lengths of time, cells exhibited a delay in subsequent division which was related to cell survival by a power function relationship. A cell was considered to be viable if it retained the ability to divide to form a colony of 50 or more cells. The components of the delay in cycle transit for viable cells heated in G1 for treatment of 20 min at 45.5° were approximately 28 hr in G1 and 20 hr in S and G2-M. This represents a 7-fold decrease in the rate progression through G1 and a 2-fold decrease through S and G2-M relative to control rates. The doubling times of viable cells, in subsequent generations, were significantly decreased to a rate 61% of that of control up to 120 h after heating. This reduction was in part due to lethal sectoring, i.e., a division which produces only one daughter that is capable of forming a viable subclone, within the viable progeny. Within a viable subclone, up to 30% of the cells that divided from 48 to 91 hr after a heat treatment of 20 min at 45.5° were found to be nonclonogenic. Following resumption of division, nonviable cells slowly lost their capacity for proliferation. Nearly all thermal tolerance development induced by a 20-min pretreatment occurred while the viable cells remained in G1. Subsequent progression into heat-sensitive S and G2-M phases modulated thermal tolerance only slightly. Finally, maximal loss of thermal tolerance was exhibited at the time corresponding to the resumption of viable cell division.
AB - Cell cycle progression delays and subsequent growth kinetics of viable and nonviable Chinese hamster cells following acute (45.5°) hyperthermia were documented in an attempt to correlate these changes with the decay of thermal tolerance. Following heating for various lengths of time, cells exhibited a delay in subsequent division which was related to cell survival by a power function relationship. A cell was considered to be viable if it retained the ability to divide to form a colony of 50 or more cells. The components of the delay in cycle transit for viable cells heated in G1 for treatment of 20 min at 45.5° were approximately 28 hr in G1 and 20 hr in S and G2-M. This represents a 7-fold decrease in the rate progression through G1 and a 2-fold decrease through S and G2-M relative to control rates. The doubling times of viable cells, in subsequent generations, were significantly decreased to a rate 61% of that of control up to 120 h after heating. This reduction was in part due to lethal sectoring, i.e., a division which produces only one daughter that is capable of forming a viable subclone, within the viable progeny. Within a viable subclone, up to 30% of the cells that divided from 48 to 91 hr after a heat treatment of 20 min at 45.5° were found to be nonclonogenic. Following resumption of division, nonviable cells slowly lost their capacity for proliferation. Nearly all thermal tolerance development induced by a 20-min pretreatment occurred while the viable cells remained in G1. Subsequent progression into heat-sensitive S and G2-M phases modulated thermal tolerance only slightly. Finally, maximal loss of thermal tolerance was exhibited at the time corresponding to the resumption of viable cell division.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021338869&partnerID=8YFLogxK
M3 - Article
C2 - 6713384
AN - SCOPUS:0021338869
VL - 44
SP - 1802
EP - 1808
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 5
ER -