CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration

Yanling Wang, Guangnan Li, Amelia Stanco, Jason E. Long, Dianna Crawford, Gregory Potter, Samuel J. Pleasure, Timothy Behrens, John L R Rubenstein

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

CXCL12/CXCR4 signaling is critical for cortical interneuron migration and their final laminar distribution. No information is yet available on CXCR7, a newly defined CXCL12 receptor. Here we demonstrated that CXCR7 regulated interneuron migration autonomously, as well as nonautonomously through its expression in immature projection neurons. Migrating cortical interneurons coexpressed Cxcr4 and Cxcr7, and Cxcr7-/- and Cxcr4-/- mutants had similar defects in interneuron positioning. Ectopic CXCL12 expression and pharmacological blockade of CXCR4 in Cxcr7-/- mutants showed that both receptors were essential for responding to CXCL12 during interneuron migration. Furthermore, live imaging revealed that Cxcr4-/- and Cxcr7-/- mutants had opposite defects in interneuron motility and leading process morphology. In vivo inhibition of Gα(i/o) signaling in migrating interneurons phenocopied the interneuron lamination defects of Cxcr4-/- mutants. On the other hand, CXCL12 stimulation of CXCR7, but not CXCR4, promoted MAP kinase signaling. Thus, we suggest that CXCR4 and CXCR7 have distinct roles and signal transduction in regulating interneuron movement and laminar positioning.

Original languageEnglish (US)
Pages (from-to)61-76
Number of pages16
JournalNeuron
Volume69
Issue number1
DOIs
StatePublished - Jan 13 2011
Externally publishedYes

Fingerprint

Interneurons
Signal Transduction
Phosphotransferases
Pharmacology
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wang, Y., Li, G., Stanco, A., Long, J. E., Crawford, D., Potter, G., ... Rubenstein, J. L. R. (2011). CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration. Neuron, 69(1), 61-76. https://doi.org/10.1016/j.neuron.2010.12.005

CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration. / Wang, Yanling; Li, Guangnan; Stanco, Amelia; Long, Jason E.; Crawford, Dianna; Potter, Gregory; Pleasure, Samuel J.; Behrens, Timothy; Rubenstein, John L R.

In: Neuron, Vol. 69, No. 1, 13.01.2011, p. 61-76.

Research output: Contribution to journalArticle

Wang, Y, Li, G, Stanco, A, Long, JE, Crawford, D, Potter, G, Pleasure, SJ, Behrens, T & Rubenstein, JLR 2011, 'CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration', Neuron, vol. 69, no. 1, pp. 61-76. https://doi.org/10.1016/j.neuron.2010.12.005
Wang Y, Li G, Stanco A, Long JE, Crawford D, Potter G et al. CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration. Neuron. 2011 Jan 13;69(1):61-76. https://doi.org/10.1016/j.neuron.2010.12.005
Wang, Yanling ; Li, Guangnan ; Stanco, Amelia ; Long, Jason E. ; Crawford, Dianna ; Potter, Gregory ; Pleasure, Samuel J. ; Behrens, Timothy ; Rubenstein, John L R. / CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration. In: Neuron. 2011 ; Vol. 69, No. 1. pp. 61-76.
@article{023eb23171fe4cb59f439294079faad2,
title = "CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration",
abstract = "CXCL12/CXCR4 signaling is critical for cortical interneuron migration and their final laminar distribution. No information is yet available on CXCR7, a newly defined CXCL12 receptor. Here we demonstrated that CXCR7 regulated interneuron migration autonomously, as well as nonautonomously through its expression in immature projection neurons. Migrating cortical interneurons coexpressed Cxcr4 and Cxcr7, and Cxcr7-/- and Cxcr4-/- mutants had similar defects in interneuron positioning. Ectopic CXCL12 expression and pharmacological blockade of CXCR4 in Cxcr7-/- mutants showed that both receptors were essential for responding to CXCL12 during interneuron migration. Furthermore, live imaging revealed that Cxcr4-/- and Cxcr7-/- mutants had opposite defects in interneuron motility and leading process morphology. In vivo inhibition of Gα(i/o) signaling in migrating interneurons phenocopied the interneuron lamination defects of Cxcr4-/- mutants. On the other hand, CXCL12 stimulation of CXCR7, but not CXCR4, promoted MAP kinase signaling. Thus, we suggest that CXCR4 and CXCR7 have distinct roles and signal transduction in regulating interneuron movement and laminar positioning.",
author = "Yanling Wang and Guangnan Li and Amelia Stanco and Long, {Jason E.} and Dianna Crawford and Gregory Potter and Pleasure, {Samuel J.} and Timothy Behrens and Rubenstein, {John L R}",
year = "2011",
month = "1",
day = "13",
doi = "10.1016/j.neuron.2010.12.005",
language = "English (US)",
volume = "69",
pages = "61--76",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - CXCR4 and CXCR7 Have Distinct Functions in Regulating Interneuron Migration

AU - Wang, Yanling

AU - Li, Guangnan

AU - Stanco, Amelia

AU - Long, Jason E.

AU - Crawford, Dianna

AU - Potter, Gregory

AU - Pleasure, Samuel J.

AU - Behrens, Timothy

AU - Rubenstein, John L R

PY - 2011/1/13

Y1 - 2011/1/13

N2 - CXCL12/CXCR4 signaling is critical for cortical interneuron migration and their final laminar distribution. No information is yet available on CXCR7, a newly defined CXCL12 receptor. Here we demonstrated that CXCR7 regulated interneuron migration autonomously, as well as nonautonomously through its expression in immature projection neurons. Migrating cortical interneurons coexpressed Cxcr4 and Cxcr7, and Cxcr7-/- and Cxcr4-/- mutants had similar defects in interneuron positioning. Ectopic CXCL12 expression and pharmacological blockade of CXCR4 in Cxcr7-/- mutants showed that both receptors were essential for responding to CXCL12 during interneuron migration. Furthermore, live imaging revealed that Cxcr4-/- and Cxcr7-/- mutants had opposite defects in interneuron motility and leading process morphology. In vivo inhibition of Gα(i/o) signaling in migrating interneurons phenocopied the interneuron lamination defects of Cxcr4-/- mutants. On the other hand, CXCL12 stimulation of CXCR7, but not CXCR4, promoted MAP kinase signaling. Thus, we suggest that CXCR4 and CXCR7 have distinct roles and signal transduction in regulating interneuron movement and laminar positioning.

AB - CXCL12/CXCR4 signaling is critical for cortical interneuron migration and their final laminar distribution. No information is yet available on CXCR7, a newly defined CXCL12 receptor. Here we demonstrated that CXCR7 regulated interneuron migration autonomously, as well as nonautonomously through its expression in immature projection neurons. Migrating cortical interneurons coexpressed Cxcr4 and Cxcr7, and Cxcr7-/- and Cxcr4-/- mutants had similar defects in interneuron positioning. Ectopic CXCL12 expression and pharmacological blockade of CXCR4 in Cxcr7-/- mutants showed that both receptors were essential for responding to CXCL12 during interneuron migration. Furthermore, live imaging revealed that Cxcr4-/- and Cxcr7-/- mutants had opposite defects in interneuron motility and leading process morphology. In vivo inhibition of Gα(i/o) signaling in migrating interneurons phenocopied the interneuron lamination defects of Cxcr4-/- mutants. On the other hand, CXCL12 stimulation of CXCR7, but not CXCR4, promoted MAP kinase signaling. Thus, we suggest that CXCR4 and CXCR7 have distinct roles and signal transduction in regulating interneuron movement and laminar positioning.

UR - http://www.scopus.com/inward/record.url?scp=78650956554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650956554&partnerID=8YFLogxK

U2 - 10.1016/j.neuron.2010.12.005

DO - 10.1016/j.neuron.2010.12.005

M3 - Article

VL - 69

SP - 61

EP - 76

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 1

ER -