CXCL13 plus interleukin 10 is highly specific for the diagnosis of CNS lymphoma

James L. Rubenstein, Valerie S. Wong, Cigall Kadoch, Hua Xin Gao, Ramon Barajas, Lingjing Chen, S. Andrew Josephson, Brian Scott, Vanja Douglas, Mekhala Maiti, Lawrence D. Kaplan, Patrick A. Treseler, Soonmee Cha, Jimmy H. Hwang, Paola Cinque, Jason G. Cyster, Clifford Lowell

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

Establishing the diagnosis of focal brain lesions in patients with unexplained neurologic symptoms represents a challenge. The goal of this study is to provide evidence supporting functional roles for CXC chemokine ligand (CXCL)13 and interleukin (IL)-10 in central nervous system (CNS) lymphomas and to evaluate the utility of each as prognostic and diagnostic biomarkers. We demonstrate for the first time that elevated CXCL13 concentration in cerebrospinal fluid (CSF) is prognostic and that CXCL13 and CXCL12 mediate chemotaxis of lymphoma cells isolated from CNS lymphoma lesions. Expression of the activated form of Janus kinase 1 supported a role for IL-10 in prosurvival signaling. We determined the concentration of CXCL13 and IL-10 in CSF of CNS lymphoma patients and control cohorts including inflammatory and degenerative neurologic disease in a multicenter study involving 220 patients. Bivariate elevated CXCL13 plus IL-10 was 99.3% specific for primary and secondary CNS lymphoma, with sensitivity significantly greater than reference standard CSF tests. These results identify CXCL13 and IL-10 as potentially important biomarkers of CNS lymphoma that merit further evaluation and support incorporation of CXCL13 and IL-10 into diagnostic algorithms for the workup of focal brain lesions in which lymphoma is a consideration.

Original languageEnglish (US)
Pages (from-to)4740-4748
Number of pages9
JournalBlood
Volume121
Issue number23
DOIs
StatePublished - Jun 6 2013

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Rubenstein, J. L., Wong, V. S., Kadoch, C., Gao, H. X., Barajas, R., Chen, L., Josephson, S. A., Scott, B., Douglas, V., Maiti, M., Kaplan, L. D., Treseler, P. A., Cha, S., Hwang, J. H., Cinque, P., Cyster, J. G., & Lowell, C. (2013). CXCL13 plus interleukin 10 is highly specific for the diagnosis of CNS lymphoma. Blood, 121(23), 4740-4748. https://doi.org/10.1182/blood-2013-01-476333