Cutting edge: Proteasome involvement in the degradation of unassembled Ig light chains

Thomas O'Hare, Gregory D. Wiens, Elizabeth A. Whitcomb, Caroline Enns, Marvin Rittenberg

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Several studies on disposal of nonsecreted Ig L chains have identified the endoplasmic reticulum as the site of degradation. Here, we examine degradation of a nonsecreted Ig L chain, T15L, and an experimentally endoplasmic reticulum-retained secretion-competent L chain, D16L, in the absence of H chains. We demonstrate that 1) degradation is specifically impaired by the proteasome-specific inhibitors carboxybenzyl-leucyl-leucyl- leucine vinyl sulfone (Z-L3VS) and lactacystin, 2) L chain degradation occurs early in the biosynthetic pathway, and 3) degradation does not require vesicular transport. Our findings indicate that previous assertions of L chain disposal within the endoplasmic reticulum must be modified. To our knowledge, we provide the first direct evidence supporting a new paradigm for removal of nonsecreted Ig L chains via dislocation to cytosolic proteasomes.

Original languageEnglish (US)
Pages (from-to)11-14
Number of pages4
JournalJournal of Immunology
Volume163
Issue number1
StatePublished - Jul 1 1999

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Proteasome Endopeptidase Complex
Endoplasmic Reticulum
Light
Proteasome Inhibitors
Biosynthetic Pathways
carboxybenzyl-leucyl-leucyl-leucine vinyl sulfone

ASJC Scopus subject areas

  • Immunology

Cite this

O'Hare, T., Wiens, G. D., Whitcomb, E. A., Enns, C., & Rittenberg, M. (1999). Cutting edge: Proteasome involvement in the degradation of unassembled Ig light chains. Journal of Immunology, 163(1), 11-14.

Cutting edge : Proteasome involvement in the degradation of unassembled Ig light chains. / O'Hare, Thomas; Wiens, Gregory D.; Whitcomb, Elizabeth A.; Enns, Caroline; Rittenberg, Marvin.

In: Journal of Immunology, Vol. 163, No. 1, 01.07.1999, p. 11-14.

Research output: Contribution to journalArticle

O'Hare, T, Wiens, GD, Whitcomb, EA, Enns, C & Rittenberg, M 1999, 'Cutting edge: Proteasome involvement in the degradation of unassembled Ig light chains', Journal of Immunology, vol. 163, no. 1, pp. 11-14.
O'Hare, Thomas ; Wiens, Gregory D. ; Whitcomb, Elizabeth A. ; Enns, Caroline ; Rittenberg, Marvin. / Cutting edge : Proteasome involvement in the degradation of unassembled Ig light chains. In: Journal of Immunology. 1999 ; Vol. 163, No. 1. pp. 11-14.
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