TY - JOUR
T1 - Cumulative Endothelial Cell Loss in Descemet Membrane Endothelial Keratoplasty Grafts From Preparation Through Insertion With Glass Injectors
AU - Downes, Kenneth
AU - Tran, Khoa D.
AU - Stoeger, Christopher G.
AU - Chamberlain, Winston
N1 - Publisher Copyright:
Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Purpose: Evaluation of cumulative Descemet membrane endothelial keratoplasty endothelial cell loss (ECL) from preparation through injection using 2 different glass injectors. Methods: Eighteen Descemet membrane endothelial keratoplasty grafts with “S” stamps were prepared by eye bank technicians. Nine grafts were assigned to injection with a modified glass Jones tube injector with a 2.4-mm opening and 9 were assigned to injection with the DORC glass pipette injector (,1.5-mm opening). The grafts were prepared and loaded into the injectors using the standard surgical technique, ejected onto a bed of viscoelastic on a glass slide, and unscrolled using viscoelastic. The grafts were stained with the vital dye Calcein-AM, then digitally imaged and analyzed using FIJI. The percentage of ECL was calculated by measuring the area of non-fluorescent pixels and dividing it by the total graft area pixels. A statistical comparison was performed using a 2-tailed unpaired t test. Results: Grafts injected using the DORC injector versus Jones tube injector had ECL of 29.2% 6 8.5% [95% confidence interval (CI)] versus 23.0% 6 5.1% (95% CI), respectively. This difference was not statistically significant (P = 0.17); however, the patterns of ECL on the grafts was different between injectors. Peripheral ECL caused by trephination and surgeon grasp sites accounted for 7.5% 6 1.2% (95% CI). Conclusions: There was no statistical difference in ECL between the 2 injectors. There were characteristic differences in patterns of ECL seen between injectors, which may be clinically relevant and indicate the types of stress that grafts are exposed to during passage through various injectors.
AB - Purpose: Evaluation of cumulative Descemet membrane endothelial keratoplasty endothelial cell loss (ECL) from preparation through injection using 2 different glass injectors. Methods: Eighteen Descemet membrane endothelial keratoplasty grafts with “S” stamps were prepared by eye bank technicians. Nine grafts were assigned to injection with a modified glass Jones tube injector with a 2.4-mm opening and 9 were assigned to injection with the DORC glass pipette injector (,1.5-mm opening). The grafts were prepared and loaded into the injectors using the standard surgical technique, ejected onto a bed of viscoelastic on a glass slide, and unscrolled using viscoelastic. The grafts were stained with the vital dye Calcein-AM, then digitally imaged and analyzed using FIJI. The percentage of ECL was calculated by measuring the area of non-fluorescent pixels and dividing it by the total graft area pixels. A statistical comparison was performed using a 2-tailed unpaired t test. Results: Grafts injected using the DORC injector versus Jones tube injector had ECL of 29.2% 6 8.5% [95% confidence interval (CI)] versus 23.0% 6 5.1% (95% CI), respectively. This difference was not statistically significant (P = 0.17); however, the patterns of ECL on the grafts was different between injectors. Peripheral ECL caused by trephination and surgeon grasp sites accounted for 7.5% 6 1.2% (95% CI). Conclusions: There was no statistical difference in ECL between the 2 injectors. There were characteristic differences in patterns of ECL seen between injectors, which may be clinically relevant and indicate the types of stress that grafts are exposed to during passage through various injectors.
KW - DMEK
KW - endothelial cell loss
KW - endothelial keratoplasty
KW - glass injector
KW - vital staining
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U2 - 10.1097/ICO.0000000000001588
DO - 10.1097/ICO.0000000000001588
M3 - Article
C2 - 29561351
AN - SCOPUS:85049103056
SN - 0277-3740
VL - 37
SP - 698
EP - 704
JO - Cornea
JF - Cornea
IS - 6
ER -