Culturing hippocampal neurons

Research output: Contribution to journalArticle

885 Citations (Scopus)

Abstract

We provide protocols for preparing low-density dissociated-cell cultures of hippocampal neurons from embryonic rats or mice. The neurons are cultured on polylysine-treated coverslips, which are suspended above an astrocyte feeder layer and maintained in serum-free medium. When cultured according to this protocol, hippocampal neurons become appropriately polarized, develop extensive axonal and dendritic arbors and form numerous, functional synaptic connections with one another. Hippocampal cultures have been used widely for visualizing the subcellular localization of endogenous or expressed proteins, for imaging protein trafficking and for defining the molecular mechanisms underlying the development of neuronal polarity, dendritic growth and synapse formation. Preparation of glial feeder cultures must begin 2 weeks in advance, and it takes 5 d to prepare coverslips as a substrate for neuronal growth. Dissecting the hippocampus and plating hippocampal neurons takes 2-3 h.

Original languageEnglish (US)
Pages (from-to)2406-2415
Number of pages10
JournalNature Protocols
Volume1
Issue number5
DOIs
StatePublished - Dec 2006

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Neurons
Body Patterning
Feeder Cells
Polylysine
Serum-Free Culture Media
Protein Transport
Growth
Cell culture
Plating
Neuroglia
Astrocytes
Synapses
Rats
Hippocampus
Proteins
Cell Culture Techniques
Imaging techniques
Substrates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Culturing hippocampal neurons. / Kaech-Petrie, Stefanie; Banker, Gary.

In: Nature Protocols, Vol. 1, No. 5, 12.2006, p. 2406-2415.

Research output: Contribution to journalArticle

Kaech-Petrie, Stefanie ; Banker, Gary. / Culturing hippocampal neurons. In: Nature Protocols. 2006 ; Vol. 1, No. 5. pp. 2406-2415.
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