Abstract
This protocol describes a culture method for supporting the maintenance and expansion of a primary mouse undifferentiated spermatogonial population containing spermatogonial stem cells (SSCs). The doubling time for SSCs in culture is relatively slow. Once established, SSC lines are split 1:2 to 1:4 every 7 d. Therefore, the time required to generate sufficient SSCs for experimentation can be considerable and requires careful planning.
Original language | English (US) |
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Pages (from-to) | 293-296 |
Number of pages | 4 |
Journal | Cold Spring Harbor Protocols |
Volume | 2017 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2017 |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology