Culture and expansion of primary undifferentiated spermatogonial stem cells

Shaun Goodyear, Ralph Brinster

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

This protocol describes a culture method for supporting the maintenance and expansion of a primary mouse undifferentiated spermatogonial population containing spermatogonial stem cells (SSCs). The doubling time for SSCs in culture is relatively slow. Once established, SSC lines are split 1:2 to 1:4 every 7 d. Therefore, the time required to generate sufficient SSCs for experimentation can be considerable and requires careful planning.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalCold Spring Harbor Protocols
Volume2017
Issue number4
DOIs
StatePublished - Apr 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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