Cubitus interruptus requires Drosophila CREB-binding protein to activate wingless expression in the Drosophila embryo

Yang Chen, Richard Goodman, Sarah M. Smolik

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

CREB-binding protein (CBP) serves as a transcriptional coactivator in multiple signal transduction pathways. The Drosophila homologue of CBP, dCBP, interacts with the transcription factors Cubitus interruptus (CI), MAD, and Dorsal (DL) and functions as a coactivator in several signaling pathways during Drosophila development, including the hedgehog (hh), decapentaplegic (dpp), and Toll pathways. Although dCBP is required for the expression of the hh target genes, wingless (wg) and patched (ptc) in vivo, and potentiates ci- mediated transcriptional activation in vitro, it is not known that ci absolutely requires dCBP for its activity. We used a yeast genetic screen to identify several ci point mutations that disrupt CI-dCBP interactions. These mutant proteins are unable to transactivate a reporter gene regulated by ci binding sites and have a lower dCBP-stimulated activity than wild-type CI. When expressed exogenously in embryos, the CI point mutants cannot activate endogenous wg expression. Furthermore, a CI mutant protein that lacks the entire dCBP interaction domain functions as a negative competitor for wild- type CI activity, and the expression of dCBP antisense RNAs can suppress CI transactivation in Kc cells. Taken together, our data suggest that dCBP function is necessary for ci-mediated transactivation of wg during Drosophila embryogenesis.

Original languageEnglish (US)
Pages (from-to)1616-1625
Number of pages10
JournalMolecular and Cellular Biology
Volume20
Issue number5
DOIs
StatePublished - Mar 2000

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CREB-Binding Protein
Drosophila Proteins
Transcriptional Activation
Drosophila
Hedgehogs
Embryonic Structures
Mutant Proteins
Antisense RNA
Reporter Genes
Point Mutation
Embryonic Development
Signal Transduction
Transcription Factors
Yeasts
Binding Sites
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cubitus interruptus requires Drosophila CREB-binding protein to activate wingless expression in the Drosophila embryo. / Chen, Yang; Goodman, Richard; Smolik, Sarah M.

In: Molecular and Cellular Biology, Vol. 20, No. 5, 03.2000, p. 1616-1625.

Research output: Contribution to journalArticle

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