CTAB enhancement of FRET in DNA structures

Taeseok Oh, Tsukasa Takahashi, Sejung Kim, Michael (Mike) Heller

Research output: Contribution to journalLetter

4 Citations (Scopus)

Abstract

The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and the other with a TexasRed acceptor, increasing the concentration of CTAB changes the fluorescence emission properties and improves the FRET transfer efficiency through changes in the polarity of the solvent, neutralization of the DNA backbone and micelle formation. For the DNA FRET system without CTAB, the DNA hybridization leads to contact quenching between TAMRA donor and TexasRed acceptor producing reduced donor emission and only a small increase in acceptor emission. At 50 μM CTAB, however, the sheathing and neutralization of the dye-conjugated dsDNA structure significantly reduces quenching by DNA bases and dye interactions, producing a large increase in FRET efficiency, which is almost four fold higher than without CTAB.

Original languageEnglish (US)
Pages (from-to)49-54
Number of pages6
JournalJournal of Biophotonics
Volume9
Issue number1-2
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Fluorescence Resonance Energy Transfer
resonance fluorescence
Bromides
bromides
DNA
deoxyribonucleic acid
energy transfer
augmentation
Coloring Agents
Dyes
dyes
strands
Quenching
quenching
fluorescence
Fluorescence Spectrometry
Fluorescence spectroscopy
Emission spectroscopy
Micelles
Dynamic light scattering

Keywords

  • CTAB
  • DNA
  • FRET
  • TAMRA
  • TexasRed

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

CTAB enhancement of FRET in DNA structures. / Oh, Taeseok; Takahashi, Tsukasa; Kim, Sejung; Heller, Michael (Mike).

In: Journal of Biophotonics, Vol. 9, No. 1-2, 01.01.2016, p. 49-54.

Research output: Contribution to journalLetter

Oh, Taeseok ; Takahashi, Tsukasa ; Kim, Sejung ; Heller, Michael (Mike). / CTAB enhancement of FRET in DNA structures. In: Journal of Biophotonics. 2016 ; Vol. 9, No. 1-2. pp. 49-54.
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