Abstract
Adeno-associated viruses are being developed as vectors for gene therapy and have been used in a number of clinical trials. Vectors to date have been based on the type species AAV-2, the structure of which was published in 2002. There is growing interest in modulating the cellular tropism and immune neutralization of AAV-2 with variants inspired by the properties of other serotypes. Towards the determination of a structure for AAV type 6, this paper reports the high-yield production, purification, crystallization and preliminary diffraction studies of infectious AAV-6 virions. The crystals diffracted to 3.2 Å resolution using synchrotron radiation. The most promising crystal form belonged to space group R3 and appeared to be suitable for initial structure determination.
Original language | English (US) |
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Pages (from-to) | 1074-1078 |
Number of pages | 5 |
Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
Volume | 64 |
Issue number | 11 |
DOIs | |
State | Published - 2008 |
Externally published | Yes |
Keywords
- Adeno-associated viruses
- Parvoviruses
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics