Crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 6

Qing Xie, Heather M. Ongley, Joan Hare, Michael Chapman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Adeno-associated viruses are being developed as vectors for gene therapy and have been used in a number of clinical trials. Vectors to date have been based on the type species AAV-2, the structure of which was published in 2002. There is growing interest in modulating the cellular tropism and immune neutralization of AAV-2 with variants inspired by the properties of other serotypes. Towards the determination of a structure for AAV type 6, this paper reports the high-yield production, purification, crystallization and preliminary diffraction studies of infectious AAV-6 virions. The crystals diffracted to 3.2 Å resolution using synchrotron radiation. The most promising crystal form belonged to space group R3 and appeared to be suitable for initial structure determination.

Original languageEnglish (US)
Pages (from-to)1074-1078
Number of pages5
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number11
DOIs
StatePublished - 2008

Fingerprint

Dependovirus
Synchrotrons
Tropism
viruses
Crystallization
Viruses
Genetic Therapy
Virion
tropism
X-Rays
Clinical Trials
Radiation
crystallization
gene therapy
Gene therapy
X rays
Crystals
Synchrotron radiation
purification
crystals

Keywords

  • Adeno-associated viruses
  • Parvoviruses

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 6. / Xie, Qing; Ongley, Heather M.; Hare, Joan; Chapman, Michael.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 64, No. 11, 2008, p. 1074-1078.

Research output: Contribution to journalArticle

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