Crystal violet reactions of coagulase negative staphylococci

R. Freeman, D. Burdess, Stephen Smith

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Twenty four reference strains and 112 clinical isolates of coagulase negative staphylococci (CNS) were examined for their reactions in the crystal violet test. Some species gave a white reaction and others a purple reaction. Results were consistent and reproducible and each species gave only one pattern of crystal violet reaction. Within the limited variety of species represented in the clinical isolates, Staphylococcus saprophyticus and S haemolyticus gave crystal violet purple reactions, in contrast to S epidermidis, which always gave a white reaction. Investigations suggested that the mechanism of the crystal violet test in S haemolyticus may be similar to that previously described in S aureus. Further work is needed to characterise the ability of crystal violet to modify S epidermidis and other central nervous system species. The crystal violet reaction, which has strong associations with invasiveness, phage group susceptibilities, colonisation persistence abilities, and nosocomial origin in S aureus may also be useful in studies of CNS disease.

Original languageEnglish (US)
Pages (from-to)283-285
Number of pages3
JournalJournal of Clinical Pathology
Volume47
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Gentian Violet
Coagulase
Staphylococcus
Staphylococcus saprophyticus
Bacteriophages
Central Nervous System

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Crystal violet reactions of coagulase negative staphylococci. / Freeman, R.; Burdess, D.; Smith, Stephen.

In: Journal of Clinical Pathology, Vol. 47, No. 3, 1994, p. 283-285.

Research output: Contribution to journalArticle

Freeman, R. ; Burdess, D. ; Smith, Stephen. / Crystal violet reactions of coagulase negative staphylococci. In: Journal of Clinical Pathology. 1994 ; Vol. 47, No. 3. pp. 283-285.
@article{fdc18cd438384ea682eaa713acc74504,
title = "Crystal violet reactions of coagulase negative staphylococci",
abstract = "Twenty four reference strains and 112 clinical isolates of coagulase negative staphylococci (CNS) were examined for their reactions in the crystal violet test. Some species gave a white reaction and others a purple reaction. Results were consistent and reproducible and each species gave only one pattern of crystal violet reaction. Within the limited variety of species represented in the clinical isolates, Staphylococcus saprophyticus and S haemolyticus gave crystal violet purple reactions, in contrast to S epidermidis, which always gave a white reaction. Investigations suggested that the mechanism of the crystal violet test in S haemolyticus may be similar to that previously described in S aureus. Further work is needed to characterise the ability of crystal violet to modify S epidermidis and other central nervous system species. The crystal violet reaction, which has strong associations with invasiveness, phage group susceptibilities, colonisation persistence abilities, and nosocomial origin in S aureus may also be useful in studies of CNS disease.",
author = "R. Freeman and D. Burdess and Stephen Smith",
year = "1994",
language = "English (US)",
volume = "47",
pages = "283--285",
journal = "Journal of Clinical Pathology - Clinical Molecular Pathology",
issn = "0021-9746",
publisher = "BMJ Publishing Group",
number = "3",

}

TY - JOUR

T1 - Crystal violet reactions of coagulase negative staphylococci

AU - Freeman, R.

AU - Burdess, D.

AU - Smith, Stephen

PY - 1994

Y1 - 1994

N2 - Twenty four reference strains and 112 clinical isolates of coagulase negative staphylococci (CNS) were examined for their reactions in the crystal violet test. Some species gave a white reaction and others a purple reaction. Results were consistent and reproducible and each species gave only one pattern of crystal violet reaction. Within the limited variety of species represented in the clinical isolates, Staphylococcus saprophyticus and S haemolyticus gave crystal violet purple reactions, in contrast to S epidermidis, which always gave a white reaction. Investigations suggested that the mechanism of the crystal violet test in S haemolyticus may be similar to that previously described in S aureus. Further work is needed to characterise the ability of crystal violet to modify S epidermidis and other central nervous system species. The crystal violet reaction, which has strong associations with invasiveness, phage group susceptibilities, colonisation persistence abilities, and nosocomial origin in S aureus may also be useful in studies of CNS disease.

AB - Twenty four reference strains and 112 clinical isolates of coagulase negative staphylococci (CNS) were examined for their reactions in the crystal violet test. Some species gave a white reaction and others a purple reaction. Results were consistent and reproducible and each species gave only one pattern of crystal violet reaction. Within the limited variety of species represented in the clinical isolates, Staphylococcus saprophyticus and S haemolyticus gave crystal violet purple reactions, in contrast to S epidermidis, which always gave a white reaction. Investigations suggested that the mechanism of the crystal violet test in S haemolyticus may be similar to that previously described in S aureus. Further work is needed to characterise the ability of crystal violet to modify S epidermidis and other central nervous system species. The crystal violet reaction, which has strong associations with invasiveness, phage group susceptibilities, colonisation persistence abilities, and nosocomial origin in S aureus may also be useful in studies of CNS disease.

UR - http://www.scopus.com/inward/record.url?scp=0028345465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028345465&partnerID=8YFLogxK

M3 - Article

C2 - 8163706

AN - SCOPUS:0028345465

VL - 47

SP - 283

EP - 285

JO - Journal of Clinical Pathology - Clinical Molecular Pathology

JF - Journal of Clinical Pathology - Clinical Molecular Pathology

SN - 0021-9746

IS - 3

ER -