Crystal structures of catalytic and regulatory subunits of rat protein kinase CK2

Wei Hong Zhou, Xiao Hong Qin, Xiao Jie Yan, Xing Qiao Xie, Liang Li, Sha Sha Fang, Jia Fu Long, John Adelman, Wei Jen Tang, Yue Quan Shen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Protein kinase CK2 consists of two catalytic subunits (CK2α) and two regulatory subunits (CK2β). Here, we report the crystal structures of rat CK2α mutant (rCK2α-ΔC, 1-335) and CK2β (rCK2β). The overall topology of rCK2α-ΔC and rCK2β are very similar to the human enzyme, although large structural differences could be observed in the N-terminal domain of rCK2α-ΔC. Our reported structure of rCK2α-ΔC is in the close conformation state while the counterpart hCK2α is in the open conformation state, indicating the conformation of CK2α molecule has high plasticity. The structure of rCK2β represents the conformation of free CK2β. Upon CK2α binding, the C-terminal region undergoes a drastic conformational change. The major region of interaction within the interface of CK2α/CK2β may serve as a bridge to transmit the conformational change and thus regulate the activity of CK2α.

Original languageEnglish (US)
Pages (from-to)220-226
Number of pages7
JournalChinese Science Bulletin
Volume54
Issue number2
DOIs
StatePublished - Jan 1 2009

Keywords

  • CK2β regulation
  • Casein kinase II
  • Catalytic subunit
  • Protein kinase CK2
  • Regulatory subunit

ASJC Scopus subject areas

  • General

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    Zhou, W. H., Qin, X. H., Yan, X. J., Xie, X. Q., Li, L., Fang, S. S., Long, J. F., Adelman, J., Tang, W. J., & Shen, Y. Q. (2009). Crystal structures of catalytic and regulatory subunits of rat protein kinase CK2. Chinese Science Bulletin, 54(2), 220-226. https://doi.org/10.1007/s11434-008-0580-2