Crystal Structure of Human Collagen XVIII Trimerization Domain: A Novel Collagen Trimerization Fold

Sergei P. Boudko, Takako Sasaki, Jürgen Engel, Thomas F. Lerch, Jay Nix, Michael Chapman, Hans Peter Bächinger

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Collagens contain a unique triple-helical structure with a repeating sequence -G-X-Y-, where proline and hydroxyproline are major constituents in X and Y positions, respectively. Folding of the collagen triple helix requires trimerization domains. Once trimerized, collagen chains are correctly aligned and the folding of the triple helix proceeds in a zipper-like fashion. Here we report the isolation, characterization, and crystal structure of the trimerization domain of human type XVIII collagen, a member of the multiplexin family. This domain differs from all other known trimerization domains in other collagens and exhibits a high trimerization potential at picomolar concentrations. Strong chain association and high specificity of binding are needed for multiplexins, which are present at very low levels.

Original languageEnglish (US)
Pages (from-to)787-802
Number of pages16
JournalJournal of Molecular Biology
Volume392
Issue number3
DOIs
StatePublished - Sep 25 2009

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Collagen Type XVIII
Collagen
Hydroxyproline
Proline

Keywords

  • collagens XVIII and XV
  • crystal structure
  • endostatin
  • non-collagenous domain
  • trimerization domain

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Crystal Structure of Human Collagen XVIII Trimerization Domain : A Novel Collagen Trimerization Fold. / Boudko, Sergei P.; Sasaki, Takako; Engel, Jürgen; Lerch, Thomas F.; Nix, Jay; Chapman, Michael; Bächinger, Hans Peter.

In: Journal of Molecular Biology, Vol. 392, No. 3, 25.09.2009, p. 787-802.

Research output: Contribution to journalArticle

Boudko, Sergei P. ; Sasaki, Takako ; Engel, Jürgen ; Lerch, Thomas F. ; Nix, Jay ; Chapman, Michael ; Bächinger, Hans Peter. / Crystal Structure of Human Collagen XVIII Trimerization Domain : A Novel Collagen Trimerization Fold. In: Journal of Molecular Biology. 2009 ; Vol. 392, No. 3. pp. 787-802.
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AU - Chapman, Michael

AU - Bächinger, Hans Peter

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