Crystal structure of Escherichia coil manganese superoxide dismutase at 2.1-Å resolution

Ross A. Edwards, Heather M. Baker, Mei M. Whittaker, James W. Whittaker, Geoffrey B. Jameson, Edward N. Baker

Research output: Contribution to journalArticle

127 Scopus citations

Abstract

The three-dimensional structure of the manganese-dependent superoxide dismutase (MnSOD) from Escherichia coli has been determined by X-ray crystallography at 2.1 Å resolution. The protein crystallizes with two homodimers in the asymmetric unit, and a model comprising 6528 protein atoms (residues 1-205 of all four monomers), four manganese ions and 415 water molecules has been refined to an R factor of 0.188 (R(free) 0.218). The structure shows a high degree of similarity with other MnSOD and FeSOD enzymes. The Mn centres are 5-coordinate, trigonal bipyramidal, with His26 and a solvent molecule, probably a hydroxide ion, as apical ligands, and His81, Asp167 and His171 as equatorial ligands. The coordinated solvent molecule is linked to a network of hydrogen bonds involving the non- coordinated carboxylate oxygen of Asp167 and a conserved glutamine residue, Gln146. The MnSOD dimer is notable for the way in which the two active sites are interconnected and a 'bridge' comprising His171 of one monomer and Glu170 of the other offers a route for inter-site communication. Comparison of E. coli MnSOD and FeSOD (a) reveals some differences in the dimer interface, (b) yields no obvious explanation for their metal specificities, and (c) provides a structural basis for differences in DNA binding, where for MnSOD the groove formed by dimerization is complementary in charge and surface contour to BDNA.

Original languageEnglish (US)
Pages (from-to)161-171
Number of pages11
JournalJournal of Biological Inorganic Chemistry
Volume3
Issue number2
DOIs
StatePublished - Apr 1 1998

Keywords

  • Crystal structure
  • DNA binding
  • Manganese enzyme
  • Metalloprotein
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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    Edwards, R. A., Baker, H. M., Whittaker, M. M., Whittaker, J. W., Jameson, G. B., & Baker, E. N. (1998). Crystal structure of Escherichia coil manganese superoxide dismutase at 2.1-Å resolution. Journal of Biological Inorganic Chemistry, 3(2), 161-171. https://doi.org/10.1007/s007750050217