Crystal structure of CTP‐ligated T state aspartate transcarbamoylase at 2.5 Å resolution: Implications for ATCase mutants and the mechanism of negative cooperativity

Richard P. Kosman, J. Eric Gouaux, William N. Lipscomb

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

The X‐ray crystal structure of CTP‐ligated T state aspartate transcarbamoylase has been refined to an R factor of 0.182 at 2.5 Å resolution using the computer program X‐PLOR. The structure contains 81 sites for solvent and has rms deviations from ideality in bond lengths and bond angles of 0.018 Å and 3.722°, respectively. The cytosine base of CTP interacts with the main chain carbonyl oxygens of rTyr‐89 and rIle‐12, the main chain NH of rIle‐12, and the amino group of rLys‐60. The ribose hydroxyls form polar contacts with the amino group of rLys‐60, a carboxylate oxygen of rAsp‐19, and the main chain carbonyl oxygen of rVal‐9. The phosphate oxygens of CTP interact with the amino group of rLys‐94, the hydroxyl of rThr‐82, and an imidazole nitrogen of rHis‐20. Recent mutagenesis experiments evaluated in parallel with the structure reported here indicate that alterations in the hydrogen bonding environment of the side chain of rAsn‐111 may be responsible for the homotropic behavior of the pAR5 mutant of ATCase. The location of the first seven residues of the regulatory chain has been identified for the first time in a refined ATCase crystal structure, and the proximity of this portion of the regulatory chain to the allosteric site suggests a potential role for these residues in nucleotide binding to the enzyme. Finally, a series of amino acid side chain rearrangements leading from the R1 CTP allosteric to the R6 CTP allosteric site has been identified which may constitute the molecular mechanism of distinct CTP binding sites on ATCase. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)147-176
Number of pages30
JournalProteins: Structure, Function, and Bioinformatics
Volume15
Issue number2
DOIs
StatePublished - Feb 1993

Keywords

  • X‐ray crystallography
  • allosteric enzyme
  • alternative amino acid conformations
  • coordinate error
  • ligand‐induced negative cooperativity
  • pAR5 mutant

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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