Crystal structure and association behaviour of the GluR2 amino-terminal domain

Rongsheng Jin, Satinder K. Singh, Shenyan Gu, Hiroyasu Furukawa, Alexander I. Sobolevsky, Jie Zhou, Yan Jin, Eric Gouaux

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Fast excitatory neurotransmission is mediated largely by ionotropic glutamate receptors (iGluRs), tetrameric, ligand-gated ion channel proteins comprised of three subfamilies, AMPA, kainate and NMDA receptors, with each subfamily sharing a common, modular-domain architecture. For all receptor subfamilies, active channels are exclusively formed by assemblages of subunits within the same subfamily, a molecular process principally encoded by the amino-terminal domain (ATD). However, the molecular basis by which the ATD guides subfamily-specific receptor assembly is not known. Here we show that AMPA receptor GluR1- and GluR2-ATDs form tightly associated dimers and, by the analysis of crystal structures of the GluR2-ATD, propose mechanisms by which the ATD guides subfamily-specific receptor assembly.

Original languageEnglish (US)
Pages (from-to)1812-1823
Number of pages12
JournalEMBO Journal
Volume28
Issue number12
DOIs
StatePublished - Jun 17 2009

Fingerprint

AMPA Receptors
Crystal structure
Ligand-Gated Ion Channels
Ionotropic Glutamate Receptors
Kainic Acid Receptors
N-Methyl-D-Aspartate Receptors
Synaptic Transmission
Dimers
Proteins

Keywords

  • Amino-terminal domain
  • GluR2
  • Glutamate receptor
  • Ion channel
  • X-ray crystallography

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Medicine(all)

Cite this

Jin, R., Singh, S. K., Gu, S., Furukawa, H., Sobolevsky, A. I., Zhou, J., ... Gouaux, E. (2009). Crystal structure and association behaviour of the GluR2 amino-terminal domain. EMBO Journal, 28(12), 1812-1823. https://doi.org/10.1038/emboj.2009.140

Crystal structure and association behaviour of the GluR2 amino-terminal domain. / Jin, Rongsheng; Singh, Satinder K.; Gu, Shenyan; Furukawa, Hiroyasu; Sobolevsky, Alexander I.; Zhou, Jie; Jin, Yan; Gouaux, Eric.

In: EMBO Journal, Vol. 28, No. 12, 17.06.2009, p. 1812-1823.

Research output: Contribution to journalArticle

Jin, R, Singh, SK, Gu, S, Furukawa, H, Sobolevsky, AI, Zhou, J, Jin, Y & Gouaux, E 2009, 'Crystal structure and association behaviour of the GluR2 amino-terminal domain', EMBO Journal, vol. 28, no. 12, pp. 1812-1823. https://doi.org/10.1038/emboj.2009.140
Jin R, Singh SK, Gu S, Furukawa H, Sobolevsky AI, Zhou J et al. Crystal structure and association behaviour of the GluR2 amino-terminal domain. EMBO Journal. 2009 Jun 17;28(12):1812-1823. https://doi.org/10.1038/emboj.2009.140
Jin, Rongsheng ; Singh, Satinder K. ; Gu, Shenyan ; Furukawa, Hiroyasu ; Sobolevsky, Alexander I. ; Zhou, Jie ; Jin, Yan ; Gouaux, Eric. / Crystal structure and association behaviour of the GluR2 amino-terminal domain. In: EMBO Journal. 2009 ; Vol. 28, No. 12. pp. 1812-1823.
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