Cryptic MBP epitope 1-20 is inducing autoimmune anterior uveitis without EAE in Lewis rats

Shuguang Jiang, Anatol Arendt, Paul A. Hargrave, Grazyna Adamus

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lewis rats immunized with myelin basic protein (MBP) developed experimental autoimmune encephalomyelitis (EAE) and associated anterior uveitis (AU). Although several cryptic epitopes of MBP have strong encephalitogenic and uveitogenic properties, the peptide corresponding to the MBP residues 1-20 was uniquely capable of inducing AU without EAE. In this study, we showed that acetylation of the N-terminal amino acid did not produce encephalitogenicity, did not enhance uveitogenicity, and did not improve T cell proliferation in Lewis rats. The cytokine production profile induced by MBP1-20 immunization was consistent with a Th1 response. In MBP-injected rats and in peptide-injected rats, the frequency of the IFN-γ-secreting cells in MBP69-89-stimulated T cells was significantly higher than the frequency of IFN-γ-secreting cells in MBP1-20-stimulated T cells. However, similar numbers of IFN-γ-producing specific cells were found in the eyes of MBP69-89 and MBP1-20 immunized rats. In these rats, the iris-infiltrating cells consisted of a much higher percentage of CD4+ T cells expressing L-selectin (CD62L) than did those cells found in the spinal cord. The results demonstrate that MBP1-20 is immunogenic and uveitogenic, although it induced only weak proliferation and weak Th1 reaction. The fact that T cells with the same specificity have different effects on target organs suggested that, in the eye and spinal cord, a distinct mechanism might mediate the recruitment of cells to these organs.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalCellular Immunology
Volume217
Issue number1-2
DOIs
StatePublished - 2002

Fingerprint

Anterior Uveitis
Autoimmune Experimental Encephalomyelitis
Epitopes
T-Lymphocytes
Myelin Basic Protein
Spinal Cord
L-Selectin
Peptides
Iris
Acetylation
myelin basic protein 1-20
Immunization
Cell Proliferation
Cytokines
Amino Acids

Keywords

  • Autoimmune disease
  • Cryptic
  • Cytokines
  • EAE
  • Eye
  • L-selectin
  • Myelin basic protein
  • Spinal cord
  • T cells
  • Uveitis

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Cryptic MBP epitope 1-20 is inducing autoimmune anterior uveitis without EAE in Lewis rats. / Jiang, Shuguang; Arendt, Anatol; Hargrave, Paul A.; Adamus, Grazyna.

In: Cellular Immunology, Vol. 217, No. 1-2, 2002, p. 87-94.

Research output: Contribution to journalArticle

Jiang, Shuguang ; Arendt, Anatol ; Hargrave, Paul A. ; Adamus, Grazyna. / Cryptic MBP epitope 1-20 is inducing autoimmune anterior uveitis without EAE in Lewis rats. In: Cellular Immunology. 2002 ; Vol. 217, No. 1-2. pp. 87-94.
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