Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts

R. R. Yeoman, B. Gerami-Naini, Shoukhrat Mitalipov, K. D. Nusser, A. A. Widmann-Browning, D. P. Wolf

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    Background: Vitrification using the cryoloop procedure was evaluated for preservation of non-human primate blastocysts by comparing survival results from two different cryoprotectant mixtures with prior results from controlled rate cooling. Methods: Rhesus monkey blastocysts were produced by intracytoplasmic sperm injection of mature oocytes from cycling females stimulated with recombinant human hormones. Morphologically well-formed blastocysts were divided between Procedure A (2.8 mol/l dimethylsulphoxide and 3.6 mol/l ethylene glycol with 0.65 mol/l sucrose and 25 μmol/l Ficoll in TALP-HEPES with 20% fetal bovine serum (TH20)) and Procedure B (3.4 mol/l glycerol and 4.5 mol/l ethylene glycol in TH20). After >48 h in liquid nitrogen, the removal of cryoprotectants was accomplished in the presence of a 3-step series of decreasing sucrose concentrations in TH20. Surviving embryos were co-cultured on buffalo rat liver cells. Results: Of 16 blastocysts vitrified via Procedure A, 38% survived with minimal lysis and only one hatched in culture; in contrast, of 33 blastocysts vitrified by Procedure B, 85% survived and 71% hatched. Of 22 blastocysts cryopreserved by conventional slow cooling, 36% survived and 6% hatched. Transfer into three recipients, each with two embryos vitrified with Procedure B, resulted in a successful twin-term pregnancy. Conclusion: Modified cryoloop vitrification with a final solution of 3.4 mol/l glycerol and 4.5 mol/l ethylene glycol is a promising procedure for preserving Rhesus monkey blastocysts that is simple, rapid, and inexpensive.

    Original languageEnglish (US)
    Pages (from-to)1965-1969
    Number of pages5
    JournalHuman Reproduction
    Volume16
    Issue number9
    StatePublished - 2001

    Fingerprint

    Vitrification
    Blastocyst
    Macaca mulatta
    Ethylene Glycol
    Glycerol
    Sucrose
    Embryonic Structures
    HEPES
    Ficoll
    Twin Pregnancy
    Intracytoplasmic Sperm Injections
    Buffaloes
    Dimethyl Sulfoxide
    Primates
    Oocytes
    Nitrogen
    Hormones
    Survival
    Liver

    Keywords

    • Blastocysts
    • Cryoloop
    • Cryopreservation
    • Monkey
    • Vitrification

    ASJC Scopus subject areas

    • Physiology
    • Developmental Biology
    • Obstetrics and Gynecology
    • Reproductive Medicine

    Cite this

    Yeoman, R. R., Gerami-Naini, B., Mitalipov, S., Nusser, K. D., Widmann-Browning, A. A., & Wolf, D. P. (2001). Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts. Human Reproduction, 16(9), 1965-1969.

    Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts. / Yeoman, R. R.; Gerami-Naini, B.; Mitalipov, Shoukhrat; Nusser, K. D.; Widmann-Browning, A. A.; Wolf, D. P.

    In: Human Reproduction, Vol. 16, No. 9, 2001, p. 1965-1969.

    Research output: Contribution to journalArticle

    Yeoman, RR, Gerami-Naini, B, Mitalipov, S, Nusser, KD, Widmann-Browning, AA & Wolf, DP 2001, 'Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts', Human Reproduction, vol. 16, no. 9, pp. 1965-1969.
    Yeoman RR, Gerami-Naini B, Mitalipov S, Nusser KD, Widmann-Browning AA, Wolf DP. Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts. Human Reproduction. 2001;16(9):1965-1969.
    Yeoman, R. R. ; Gerami-Naini, B. ; Mitalipov, Shoukhrat ; Nusser, K. D. ; Widmann-Browning, A. A. ; Wolf, D. P. / Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts. In: Human Reproduction. 2001 ; Vol. 16, No. 9. pp. 1965-1969.
    @article{11c71ab17ae24dd5b3ec4ac255deac37,
    title = "Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts",
    abstract = "Background: Vitrification using the cryoloop procedure was evaluated for preservation of non-human primate blastocysts by comparing survival results from two different cryoprotectant mixtures with prior results from controlled rate cooling. Methods: Rhesus monkey blastocysts were produced by intracytoplasmic sperm injection of mature oocytes from cycling females stimulated with recombinant human hormones. Morphologically well-formed blastocysts were divided between Procedure A (2.8 mol/l dimethylsulphoxide and 3.6 mol/l ethylene glycol with 0.65 mol/l sucrose and 25 μmol/l Ficoll in TALP-HEPES with 20{\%} fetal bovine serum (TH20)) and Procedure B (3.4 mol/l glycerol and 4.5 mol/l ethylene glycol in TH20). After >48 h in liquid nitrogen, the removal of cryoprotectants was accomplished in the presence of a 3-step series of decreasing sucrose concentrations in TH20. Surviving embryos were co-cultured on buffalo rat liver cells. Results: Of 16 blastocysts vitrified via Procedure A, 38{\%} survived with minimal lysis and only one hatched in culture; in contrast, of 33 blastocysts vitrified by Procedure B, 85{\%} survived and 71{\%} hatched. Of 22 blastocysts cryopreserved by conventional slow cooling, 36{\%} survived and 6{\%} hatched. Transfer into three recipients, each with two embryos vitrified with Procedure B, resulted in a successful twin-term pregnancy. Conclusion: Modified cryoloop vitrification with a final solution of 3.4 mol/l glycerol and 4.5 mol/l ethylene glycol is a promising procedure for preserving Rhesus monkey blastocysts that is simple, rapid, and inexpensive.",
    keywords = "Blastocysts, Cryoloop, Cryopreservation, Monkey, Vitrification",
    author = "Yeoman, {R. R.} and B. Gerami-Naini and Shoukhrat Mitalipov and Nusser, {K. D.} and Widmann-Browning, {A. A.} and Wolf, {D. P.}",
    year = "2001",
    language = "English (US)",
    volume = "16",
    pages = "1965--1969",
    journal = "Human Reproduction",
    issn = "0268-1161",
    publisher = "Oxford University Press",
    number = "9",

    }

    TY - JOUR

    T1 - Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts

    AU - Yeoman, R. R.

    AU - Gerami-Naini, B.

    AU - Mitalipov, Shoukhrat

    AU - Nusser, K. D.

    AU - Widmann-Browning, A. A.

    AU - Wolf, D. P.

    PY - 2001

    Y1 - 2001

    N2 - Background: Vitrification using the cryoloop procedure was evaluated for preservation of non-human primate blastocysts by comparing survival results from two different cryoprotectant mixtures with prior results from controlled rate cooling. Methods: Rhesus monkey blastocysts were produced by intracytoplasmic sperm injection of mature oocytes from cycling females stimulated with recombinant human hormones. Morphologically well-formed blastocysts were divided between Procedure A (2.8 mol/l dimethylsulphoxide and 3.6 mol/l ethylene glycol with 0.65 mol/l sucrose and 25 μmol/l Ficoll in TALP-HEPES with 20% fetal bovine serum (TH20)) and Procedure B (3.4 mol/l glycerol and 4.5 mol/l ethylene glycol in TH20). After >48 h in liquid nitrogen, the removal of cryoprotectants was accomplished in the presence of a 3-step series of decreasing sucrose concentrations in TH20. Surviving embryos were co-cultured on buffalo rat liver cells. Results: Of 16 blastocysts vitrified via Procedure A, 38% survived with minimal lysis and only one hatched in culture; in contrast, of 33 blastocysts vitrified by Procedure B, 85% survived and 71% hatched. Of 22 blastocysts cryopreserved by conventional slow cooling, 36% survived and 6% hatched. Transfer into three recipients, each with two embryos vitrified with Procedure B, resulted in a successful twin-term pregnancy. Conclusion: Modified cryoloop vitrification with a final solution of 3.4 mol/l glycerol and 4.5 mol/l ethylene glycol is a promising procedure for preserving Rhesus monkey blastocysts that is simple, rapid, and inexpensive.

    AB - Background: Vitrification using the cryoloop procedure was evaluated for preservation of non-human primate blastocysts by comparing survival results from two different cryoprotectant mixtures with prior results from controlled rate cooling. Methods: Rhesus monkey blastocysts were produced by intracytoplasmic sperm injection of mature oocytes from cycling females stimulated with recombinant human hormones. Morphologically well-formed blastocysts were divided between Procedure A (2.8 mol/l dimethylsulphoxide and 3.6 mol/l ethylene glycol with 0.65 mol/l sucrose and 25 μmol/l Ficoll in TALP-HEPES with 20% fetal bovine serum (TH20)) and Procedure B (3.4 mol/l glycerol and 4.5 mol/l ethylene glycol in TH20). After >48 h in liquid nitrogen, the removal of cryoprotectants was accomplished in the presence of a 3-step series of decreasing sucrose concentrations in TH20. Surviving embryos were co-cultured on buffalo rat liver cells. Results: Of 16 blastocysts vitrified via Procedure A, 38% survived with minimal lysis and only one hatched in culture; in contrast, of 33 blastocysts vitrified by Procedure B, 85% survived and 71% hatched. Of 22 blastocysts cryopreserved by conventional slow cooling, 36% survived and 6% hatched. Transfer into three recipients, each with two embryos vitrified with Procedure B, resulted in a successful twin-term pregnancy. Conclusion: Modified cryoloop vitrification with a final solution of 3.4 mol/l glycerol and 4.5 mol/l ethylene glycol is a promising procedure for preserving Rhesus monkey blastocysts that is simple, rapid, and inexpensive.

    KW - Blastocysts

    KW - Cryoloop

    KW - Cryopreservation

    KW - Monkey

    KW - Vitrification

    UR - http://www.scopus.com/inward/record.url?scp=0034807949&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0034807949&partnerID=8YFLogxK

    M3 - Article

    VL - 16

    SP - 1965

    EP - 1969

    JO - Human Reproduction

    JF - Human Reproduction

    SN - 0268-1161

    IS - 9

    ER -