Cross-talk between membrane-initiated and nuclear-initiated oestrogen signalling in the hypothalamus

T. A. Roepke, Jian Qiu, M. A. Bosch, Oline Ronnekleiv, Martin Kelly

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

It is increasingly evident that 17β-oestradiol (E2), via a distinct membrane oestrogen receptor (Gq-mER), can rapidly activate kinase pathways to have multiple downstream actions in central nervous system (CNS) neurones. We have found that E2 can rapidly reduce the potency of the GABAB receptor agonist baclofen and mu-opioid receptor agonist DAMGO to activate G-protein-coupled, inwardly rectifying K+ (GIRK) channels in hypothalamic neurones, thereby increasing the excitability (firing activity) of pro-opiomelanocortin (POMC) and dopamine neurones. These effects are mimicked by the membrane impermeant E2-BSA and a new ligand (STX) that is selective for the Gq-mER that does not bind to ERα or ERβ. Both E2 and STX are fully efficacious in attenuating the GABA B response in ERα, ERβ and GPR 30 knockout mice in an ICI 182780 reversible manner. These findings are further proof that E2 signals through a unique plasma membrane ER. We have characterised the coupling of this Gq-mER to a Gq-mediated activation of phospholipase C leading to the up-regulation of protein kinase Cδ and protein kinase A activity in these neurones, which ultimately alters gene transcription. Finally, as proof of principle, we have found that STX, similar to E2, reduces food intake and body weight gain in ovariectomised females. STX, presumably via the Gq-mER, also regulates gene expression of a number of relevant targets including cation channels and signalling molecules that are critical for regulating (as a prime example) POMC neuronal excitability. Therefore, E2 can activate multiple receptor-mediated pathways to modulate excitability and gene transcription in CNS neurones that are critical for controlling homeostasis and motivated behaviors.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalJournal of Neuroendocrinology
Volume21
Issue number4
DOIs
StatePublished - 2009

Fingerprint

Nuclear Envelope
Hypothalamus
Estrogens
Neurons
Pro-Opiomelanocortin
Central Nervous System
Ala(2)-MePhe(4)-Gly(5)-enkephalin
GABA-B Receptors
Inwardly Rectifying Potassium Channel
Membranes
mu Opioid Receptor
Dopaminergic Neurons
Type C Phospholipases
Cyclic AMP-Dependent Protein Kinases
GTP-Binding Proteins
Knockout Mice
Estrogen Receptors
gamma-Aminobutyric Acid
Genes
Weight Gain

Keywords

  • ER
  • Gene expression
  • Gq-mER
  • Hypothalamus
  • Signalling pathways

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

Cross-talk between membrane-initiated and nuclear-initiated oestrogen signalling in the hypothalamus. / Roepke, T. A.; Qiu, Jian; Bosch, M. A.; Ronnekleiv, Oline; Kelly, Martin.

In: Journal of Neuroendocrinology, Vol. 21, No. 4, 2009, p. 263-270.

Research output: Contribution to journalArticle

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