Cross-reactivity of rat, mouse, and human IgD

H. Golding, M. A. Cuchens, G. A. Leslie, M. B. Rittenberg

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Highly purified sheep anti-rat lymphocyte membrane IgD (mIgD) was used to detect cross-reactivity with the putative murine-δ chain on mouse lymphocytes. Cross-reactivity is demonstrated by indirect immunofluorescent staining and by immunoprecipitation of 125I-labeled lymphocyte membrane extracts followed by electrophoresis on 10% polyacrylamide gels. In addition, cross-reactivity of anti-rat-δ with human IgD is shown by gel diffusion analysis. The anti-rat-δ reagent stained both Ig5a+ and Ig5b+ lymphocytes. Preincubation of Ig5b+ (but not Ig5a+) cells with monoclonal allotype-specific antibodies (anti-Ig5b) under capping conditions caused inhibition of staining by the sheep anti-rat-δ reagent, indicating that it is the δ-chain that is recognized on mouse lymphocytes and that the anti-rat-δ reagent does not distinguish between mouse-δ allotypes. Furthermore, absorption of the sheep anti-rat-δ serum with purified human IgD reduced subsequent staining of mouse lymphocytes by approximately 50%; staining was not affected by absorption with human IgM. This xenogeneic anti-δ antiserum appears to detect determinants on the δ-heavy chain, which are shared by at least three species of mammals, suggesting that these determinants represent important molecular features conserved during evolution.

Original languageEnglish (US)
Pages (from-to)2751-2756
Number of pages6
JournalJournal of Immunology
Issue number6
StatePublished - Dec 1 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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