Critical roles of ARHGAP36 as a signal transduction mediator of shh pathway in lateral motor columnar specification

Heejin Nam, Shin Jeon, Hyejin An, Jaeyoung Yoo, Hyo Jong Lee, Soo Kyung Lee, Seunghee Lee

Research output: Contribution to journalArticlepeer-review

Abstract

During spinal cord development, Sonic hedgehog (Shh), secreted from the floor plate, plays an important role in the production of motor neurons by patterning the ventral neural tube, which establishes MN progenitor identity. It remains unknown, however, if Shh signaling plays a role in generating columnar diversity of MNs that connect distinct target muscles. Here, we report that Shh, expressed in MNs, is essential for the formation of lateral motor column (LMC) neurons in vertebrate spinal cord. This novel activity of Shh is mediated by its downstream effector ARHGAP36, whose expression is directly induced by the MN-specific transcription factor complex Isl1-Lhx3. Furthermore, we found that AKT stimulates the Shh activity to induce LMC MNs through the stabilization of ARHGAP36 proteins. Taken together, our data reveal that Shh, secreted from MNs, plays a crucial role in generating MN diversity via a regulatory axis of Shh-AKTARHGAP36 in the developing mouse spinal cord.

Original languageEnglish (US)
Article numbere46683
JournaleLife
Volume8
DOIs
StatePublished - Jul 2019

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Critical roles of ARHGAP36 as a signal transduction mediator of shh pathway in lateral motor columnar specification'. Together they form a unique fingerprint.

Cite this