CRISPR-cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired Enterococcus faecalis

Katie M. Burley, Christine Sedgley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Introduction: Microorganisms are vulnerable to invasion by mobile genetic elements such as viruses, plasmids, and transposons. The recently discovered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated system, or CRISPR-Cas, is an adaptive immunity system found in most archaea and many bacteria that targets and inactivates invading foreign genetic elements. Cells with CRISPR-cas are more likely to resist the invasion and uptake of foreign DNA such as viruses, plasmids, and transposons. The aims of this study were to (1) compare the occurrence of CRISPR-cas in collections of endodontic (n = 34), oral (n = 21), and multidrug-resistant hospital-acquired strains of Enterococcus faecalis (n = 23) and (2) evaluate the distribution of antibiotic resistance and virulence traits among strains without CRISPR-cas. Methods: E. faecalis strains were screened for CRISPR1-cas and CRISPR3-cas by using polymerase chain reaction, and products were verified by DNA sequencing. Associations were investigated between the occurrence of CRISPR-cas and the expression of phenotypic traits (antibiotic resistance, gelatinase activity, bacteriocin production, hemolysin activity, and clumping response to pheromone). Results: CRISPR-cas determinants were present in proportionally more endodontic (25 of 34) and oral (15 of 21) strains than hospital-acquired (9 of 23) strains (P =.01 and.04, respectively). Significant associations were found between the absence of CRISPR-cas and the presence of antibiotic resistance in strains overall (P =.04) and bacteriocin activity in endodontic strains (P =.01). Conclusions: Evidence for the presence of CRISPR-cas in the majority of endodontic and oral E. faecalis strains raises intriguing questions as to how prokaryotic immune systems might modulate interactions within the polymicrobial endodontic biofilm environment.

Original languageEnglish (US)
Pages (from-to)1511-1515
Number of pages5
JournalJournal of Endodontics
Volume38
Issue number11
DOIs
StatePublished - Nov 2012

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Enterococcus faecalis
Endodontics
Immune System
Microbial Drug Resistance
Bacteriocins
Plasmids
Interspersed Repetitive Sequences
Viruses
Gelatinases
Hemolysin Proteins
Pheromones
Archaea
Adaptive Immunity
Biofilms
DNA Sequence Analysis
Virulence

Keywords

  • CRISPR-cas
  • endodontic
  • Enterococcus faecalis
  • multidrug-resistant hospital-acquired bacteria
  • oral

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

CRISPR-cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired Enterococcus faecalis. / Burley, Katie M.; Sedgley, Christine.

In: Journal of Endodontics, Vol. 38, No. 11, 11.2012, p. 1511-1515.

Research output: Contribution to journalArticle

@article{c4730661b9424b959a010f7decb50de6,
title = "CRISPR-cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired Enterococcus faecalis",
abstract = "Introduction: Microorganisms are vulnerable to invasion by mobile genetic elements such as viruses, plasmids, and transposons. The recently discovered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated system, or CRISPR-Cas, is an adaptive immunity system found in most archaea and many bacteria that targets and inactivates invading foreign genetic elements. Cells with CRISPR-cas are more likely to resist the invasion and uptake of foreign DNA such as viruses, plasmids, and transposons. The aims of this study were to (1) compare the occurrence of CRISPR-cas in collections of endodontic (n = 34), oral (n = 21), and multidrug-resistant hospital-acquired strains of Enterococcus faecalis (n = 23) and (2) evaluate the distribution of antibiotic resistance and virulence traits among strains without CRISPR-cas. Methods: E. faecalis strains were screened for CRISPR1-cas and CRISPR3-cas by using polymerase chain reaction, and products were verified by DNA sequencing. Associations were investigated between the occurrence of CRISPR-cas and the expression of phenotypic traits (antibiotic resistance, gelatinase activity, bacteriocin production, hemolysin activity, and clumping response to pheromone). Results: CRISPR-cas determinants were present in proportionally more endodontic (25 of 34) and oral (15 of 21) strains than hospital-acquired (9 of 23) strains (P =.01 and.04, respectively). Significant associations were found between the absence of CRISPR-cas and the presence of antibiotic resistance in strains overall (P =.04) and bacteriocin activity in endodontic strains (P =.01). Conclusions: Evidence for the presence of CRISPR-cas in the majority of endodontic and oral E. faecalis strains raises intriguing questions as to how prokaryotic immune systems might modulate interactions within the polymicrobial endodontic biofilm environment.",
keywords = "CRISPR-cas, endodontic, Enterococcus faecalis, multidrug-resistant hospital-acquired bacteria, oral",
author = "Burley, {Katie M.} and Christine Sedgley",
year = "2012",
month = "11",
doi = "10.1016/j.joen.2012.07.004",
language = "English (US)",
volume = "38",
pages = "1511--1515",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "11",

}

TY - JOUR

T1 - CRISPR-cas, a prokaryotic adaptive immune system, in endodontic, oral, and multidrug-resistant hospital-acquired Enterococcus faecalis

AU - Burley, Katie M.

AU - Sedgley, Christine

PY - 2012/11

Y1 - 2012/11

N2 - Introduction: Microorganisms are vulnerable to invasion by mobile genetic elements such as viruses, plasmids, and transposons. The recently discovered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated system, or CRISPR-Cas, is an adaptive immunity system found in most archaea and many bacteria that targets and inactivates invading foreign genetic elements. Cells with CRISPR-cas are more likely to resist the invasion and uptake of foreign DNA such as viruses, plasmids, and transposons. The aims of this study were to (1) compare the occurrence of CRISPR-cas in collections of endodontic (n = 34), oral (n = 21), and multidrug-resistant hospital-acquired strains of Enterococcus faecalis (n = 23) and (2) evaluate the distribution of antibiotic resistance and virulence traits among strains without CRISPR-cas. Methods: E. faecalis strains were screened for CRISPR1-cas and CRISPR3-cas by using polymerase chain reaction, and products were verified by DNA sequencing. Associations were investigated between the occurrence of CRISPR-cas and the expression of phenotypic traits (antibiotic resistance, gelatinase activity, bacteriocin production, hemolysin activity, and clumping response to pheromone). Results: CRISPR-cas determinants were present in proportionally more endodontic (25 of 34) and oral (15 of 21) strains than hospital-acquired (9 of 23) strains (P =.01 and.04, respectively). Significant associations were found between the absence of CRISPR-cas and the presence of antibiotic resistance in strains overall (P =.04) and bacteriocin activity in endodontic strains (P =.01). Conclusions: Evidence for the presence of CRISPR-cas in the majority of endodontic and oral E. faecalis strains raises intriguing questions as to how prokaryotic immune systems might modulate interactions within the polymicrobial endodontic biofilm environment.

AB - Introduction: Microorganisms are vulnerable to invasion by mobile genetic elements such as viruses, plasmids, and transposons. The recently discovered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated system, or CRISPR-Cas, is an adaptive immunity system found in most archaea and many bacteria that targets and inactivates invading foreign genetic elements. Cells with CRISPR-cas are more likely to resist the invasion and uptake of foreign DNA such as viruses, plasmids, and transposons. The aims of this study were to (1) compare the occurrence of CRISPR-cas in collections of endodontic (n = 34), oral (n = 21), and multidrug-resistant hospital-acquired strains of Enterococcus faecalis (n = 23) and (2) evaluate the distribution of antibiotic resistance and virulence traits among strains without CRISPR-cas. Methods: E. faecalis strains were screened for CRISPR1-cas and CRISPR3-cas by using polymerase chain reaction, and products were verified by DNA sequencing. Associations were investigated between the occurrence of CRISPR-cas and the expression of phenotypic traits (antibiotic resistance, gelatinase activity, bacteriocin production, hemolysin activity, and clumping response to pheromone). Results: CRISPR-cas determinants were present in proportionally more endodontic (25 of 34) and oral (15 of 21) strains than hospital-acquired (9 of 23) strains (P =.01 and.04, respectively). Significant associations were found between the absence of CRISPR-cas and the presence of antibiotic resistance in strains overall (P =.04) and bacteriocin activity in endodontic strains (P =.01). Conclusions: Evidence for the presence of CRISPR-cas in the majority of endodontic and oral E. faecalis strains raises intriguing questions as to how prokaryotic immune systems might modulate interactions within the polymicrobial endodontic biofilm environment.

KW - CRISPR-cas

KW - endodontic

KW - Enterococcus faecalis

KW - multidrug-resistant hospital-acquired bacteria

KW - oral

UR - http://www.scopus.com/inward/record.url?scp=84867576666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867576666&partnerID=8YFLogxK

U2 - 10.1016/j.joen.2012.07.004

DO - 10.1016/j.joen.2012.07.004

M3 - Article

C2 - 23063226

AN - SCOPUS:84867576666

VL - 38

SP - 1511

EP - 1515

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 11

ER -