Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle

Suresh I. Prajapati, Carlo O. Martinez, Jinu Abraham, Amanda T. Mccleish, Joel E. Michalek, Linda M. Mcmanus, Brian P. Rubin, Paula K. Shireman, Charles Keller

Research output: Contribution to journalArticle

Abstract

The near-infrared wavelengths (700-900 nm) are the most suitable optical window for light penetration and deep tissue imaging in small animals. Herein we report a near-infrared fluorescent contrast agent, crimson carrier, which acts as a blood pool contrast agent to detect and quantify injury and disease in live animals. After determining the excitation-emission spectra and pharmacokinetics, crimson carrier was injected into myoinjured mice to monitor their recovery. Crimson carrier was also used to image transgenic mice with spontaneous tumors. Crimson carrier has maximal excitation and emission wavelengths of 745 nm and 820 nm, respectively. Elimination occurs predominantly via urinary excretion. We demonstrate the utility of this contrast agent for serial imaging of traumatized muscle as well as muscle tumors. The unique long-acting pharmacokinetics and urinary excretion route characteristics make crimson carrier a contrast agent of choice for the visualization of tumors and injured muscle or other tissues in live animal studies.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalMuscle and Nerve
Volume42
Issue number2
DOIs
StatePublished - Aug 2010
Externally publishedYes

Fingerprint

Contrast Media
Muscles
Pharmacokinetics
Neoplasms
Fluorescent Dyes
Transgenic Mice
Light
Wounds and Injuries

Keywords

  • Crimson carrier
  • Muscle injury
  • Near-infrared contrast agent
  • Optical imaging
  • Tumor imaging

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)
  • Physiology

Cite this

Prajapati, S. I., Martinez, C. O., Abraham, J., Mccleish, A. T., Michalek, J. E., Mcmanus, L. M., ... Keller, C. (2010). Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle. Muscle and Nerve, 42(2), 245-251. https://doi.org/10.1002/mus.21682

Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle. / Prajapati, Suresh I.; Martinez, Carlo O.; Abraham, Jinu; Mccleish, Amanda T.; Michalek, Joel E.; Mcmanus, Linda M.; Rubin, Brian P.; Shireman, Paula K.; Keller, Charles.

In: Muscle and Nerve, Vol. 42, No. 2, 08.2010, p. 245-251.

Research output: Contribution to journalArticle

Prajapati, SI, Martinez, CO, Abraham, J, Mccleish, AT, Michalek, JE, Mcmanus, LM, Rubin, BP, Shireman, PK & Keller, C 2010, 'Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle', Muscle and Nerve, vol. 42, no. 2, pp. 245-251. https://doi.org/10.1002/mus.21682
Prajapati SI, Martinez CO, Abraham J, Mccleish AT, Michalek JE, Mcmanus LM et al. Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle. Muscle and Nerve. 2010 Aug;42(2):245-251. https://doi.org/10.1002/mus.21682
Prajapati, Suresh I. ; Martinez, Carlo O. ; Abraham, Jinu ; Mccleish, Amanda T. ; Michalek, Joel E. ; Mcmanus, Linda M. ; Rubin, Brian P. ; Shireman, Paula K. ; Keller, Charles. / Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle. In: Muscle and Nerve. 2010 ; Vol. 42, No. 2. pp. 245-251.
@article{73fb27def57f4a19bac42e617e724c00,
title = "Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle",
abstract = "The near-infrared wavelengths (700-900 nm) are the most suitable optical window for light penetration and deep tissue imaging in small animals. Herein we report a near-infrared fluorescent contrast agent, crimson carrier, which acts as a blood pool contrast agent to detect and quantify injury and disease in live animals. After determining the excitation-emission spectra and pharmacokinetics, crimson carrier was injected into myoinjured mice to monitor their recovery. Crimson carrier was also used to image transgenic mice with spontaneous tumors. Crimson carrier has maximal excitation and emission wavelengths of 745 nm and 820 nm, respectively. Elimination occurs predominantly via urinary excretion. We demonstrate the utility of this contrast agent for serial imaging of traumatized muscle as well as muscle tumors. The unique long-acting pharmacokinetics and urinary excretion route characteristics make crimson carrier a contrast agent of choice for the visualization of tumors and injured muscle or other tissues in live animal studies.",
keywords = "Crimson carrier, Muscle injury, Near-infrared contrast agent, Optical imaging, Tumor imaging",
author = "Prajapati, {Suresh I.} and Martinez, {Carlo O.} and Jinu Abraham and Mccleish, {Amanda T.} and Michalek, {Joel E.} and Mcmanus, {Linda M.} and Rubin, {Brian P.} and Shireman, {Paula K.} and Charles Keller",
year = "2010",
month = "8",
doi = "10.1002/mus.21682",
language = "English (US)",
volume = "42",
pages = "245--251",
journal = "Muscle and Nerve",
issn = "0148-639X",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Crimson carrier, a long-acting contrast agent for in vivo near-infrared imaging of injured and diseased muscle

AU - Prajapati, Suresh I.

AU - Martinez, Carlo O.

AU - Abraham, Jinu

AU - Mccleish, Amanda T.

AU - Michalek, Joel E.

AU - Mcmanus, Linda M.

AU - Rubin, Brian P.

AU - Shireman, Paula K.

AU - Keller, Charles

PY - 2010/8

Y1 - 2010/8

N2 - The near-infrared wavelengths (700-900 nm) are the most suitable optical window for light penetration and deep tissue imaging in small animals. Herein we report a near-infrared fluorescent contrast agent, crimson carrier, which acts as a blood pool contrast agent to detect and quantify injury and disease in live animals. After determining the excitation-emission spectra and pharmacokinetics, crimson carrier was injected into myoinjured mice to monitor their recovery. Crimson carrier was also used to image transgenic mice with spontaneous tumors. Crimson carrier has maximal excitation and emission wavelengths of 745 nm and 820 nm, respectively. Elimination occurs predominantly via urinary excretion. We demonstrate the utility of this contrast agent for serial imaging of traumatized muscle as well as muscle tumors. The unique long-acting pharmacokinetics and urinary excretion route characteristics make crimson carrier a contrast agent of choice for the visualization of tumors and injured muscle or other tissues in live animal studies.

AB - The near-infrared wavelengths (700-900 nm) are the most suitable optical window for light penetration and deep tissue imaging in small animals. Herein we report a near-infrared fluorescent contrast agent, crimson carrier, which acts as a blood pool contrast agent to detect and quantify injury and disease in live animals. After determining the excitation-emission spectra and pharmacokinetics, crimson carrier was injected into myoinjured mice to monitor their recovery. Crimson carrier was also used to image transgenic mice with spontaneous tumors. Crimson carrier has maximal excitation and emission wavelengths of 745 nm and 820 nm, respectively. Elimination occurs predominantly via urinary excretion. We demonstrate the utility of this contrast agent for serial imaging of traumatized muscle as well as muscle tumors. The unique long-acting pharmacokinetics and urinary excretion route characteristics make crimson carrier a contrast agent of choice for the visualization of tumors and injured muscle or other tissues in live animal studies.

KW - Crimson carrier

KW - Muscle injury

KW - Near-infrared contrast agent

KW - Optical imaging

KW - Tumor imaging

UR - http://www.scopus.com/inward/record.url?scp=77955168917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955168917&partnerID=8YFLogxK

U2 - 10.1002/mus.21682

DO - 10.1002/mus.21682

M3 - Article

C2 - 20544935

AN - SCOPUS:77955168917

VL - 42

SP - 245

EP - 251

JO - Muscle and Nerve

JF - Muscle and Nerve

SN - 0148-639X

IS - 2

ER -