Creation of fibrinogen-enhanced experimental blood clots to evaluate mechanical thrombectomy devices for treatment of acute stroke: An in vitro study

Zhong Hua Luo, Alex Chung, Gibok Choi, Yih Huie Lin, Barry Uchida, Dusan Pavcnik, Marc Loriaux, Gary Nesbit, Frederick Keller, Josef Rosch

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: To explore if addition of fibrinogen to the most commonly used experimental blood clot (EBC) model would improve its mechanical properties and histologic structure. Materials and Methods: Fresh blood from three swine was used to create four EBC types. The Gralla model of thrombin-induced barium-opaque EBC served as the control. In three other EBC types, 50 mg, 100 mg, and 200 mg of bovine fibrinogen were added. Evaluation of EBCs was done with three tests: manual elongation, injection through an 8-F catheter, and an opacity test. Thirty EBCs of each type were evaluated with each test. Histologic evaluation followed. Results: The control EBCs had low tensile strength and broke at 165% elongation. However, they were elastic and returned to their original length after catheter injection. The EBCs with fibrinogen exhibited increased tensile strength with increasing fibrinogen doses and withstood elongation to 213% (P

Original languageEnglish (US)
Pages (from-to)1077-1083
Number of pages7
JournalJournal of Vascular and Interventional Radiology
Volume23
Issue number8
DOIs
StatePublished - Aug 2012

Fingerprint

Thrombectomy
Fibrinogen
Thrombosis
Stroke
Equipment and Supplies
Tensile Strength
Catheters
Injections
Barium
Therapeutics
Thrombin
Swine
In Vitro Techniques

Keywords

  • EBC
  • experimental blood clot
  • mechanical thrombectomy device
  • MTD

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Creation of fibrinogen-enhanced experimental blood clots to evaluate mechanical thrombectomy devices for treatment of acute stroke : An in vitro study. / Luo, Zhong Hua; Chung, Alex; Choi, Gibok; Lin, Yih Huie; Uchida, Barry; Pavcnik, Dusan; Loriaux, Marc; Nesbit, Gary; Keller, Frederick; Rosch, Josef.

In: Journal of Vascular and Interventional Radiology, Vol. 23, No. 8, 08.2012, p. 1077-1083.

Research output: Contribution to journalArticle

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