Cre activated and inactivated recombinant adeno-associated viral vectors for neuronal anatomical tracing or activity manipulation

Arpiar Saunders, Bernardo L. Sabatini

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Recombinant adeno-associated viruses (rAAVs) transcriptionally activated by Cre recombinase (Cre-On) are powerful tools for determining the anatomy and function of genetically defined neuronal types in transgenic Cre driver mice. Here we describe how rAAVs transcriptionally inactivated by Cre (Cre-Off) can be used in conjunction with Cre-On rAAVs or genomic Cre-reporter alleles to study brain circuits. Intracranial injection of Cre-On/Cre-OffrAAVs into spatially intermingled Cre+ and Cre- neurons allows these populations to be differentially labeled or manipulated within individual animals. This comparison helps define the unique properties of Cre+ neurons, highlighting the specialized role they play in their constituent brain circuits. This protocol touches on the conceptual and experimental background of Cre-OffrAAV systems, including caveats and methods of validation.

Original languageEnglish (US)
Pages (from-to)1.24.1-1.24.15
JournalCurrent Protocols in Neuroscience
Volume2015
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • AAV
  • Cre-dependent virus
  • Cre-lox
  • RAAV
  • Viral tracer

ASJC Scopus subject areas

  • Neuroscience(all)

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