Coupled secretion of chloride and mucus in skin of Xenopus laevis: Possible role for CFTR

J. F. Engelhardt, S. S. Smith, E. Allen, J. R. Yankaskas, D. C. Dawson, J. M. Wilson

Research output: Contribution to journalArticle

Abstract

We used the isolated skin of Xenopus laevis to investigate the relationship between the secretion of salt, water, and mucus by submucosal glands expressing the cystic fibrosis transmembrane conductance regulator (CFTR). In situ hybridization and immunofluorescence provided evidence for specific expression of CFTR in the mucus-secreting cells of the subepidermal glands. Stimulation of isolated sheets of skin with 8-(4-chlorophenylthio)- adenosine 3',5'-cyclic monophosphate produced active Cl secretion and a marked increase in tissue conductance that was correlated with mucous cell degranulation and the distention of the glandular ducts. This coordinated increase in active secretion of salt and mucus was abolished by pretreatment of skins with bumetanide or by removing Cl from the bathing solutions. These results provide evidence for an intimate coupling between electrolyte transport and mucus secretion that may illuminate the pathophysiology of mucus-producing glands in cystic fibrosis lung disease.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume267
Issue number2 36-2
StatePublished - 1994
Externally publishedYes

Fingerprint

Cystic Fibrosis Transmembrane Conductance Regulator
Xenopus laevis
Mucus
Chlorides
Skin
Bumetanide
Pulmonary diseases
Saline water
Cyclic AMP
Ducts
Salts
Electrolytes
Cell Degranulation
Tissue
Cystic Fibrosis
Lung Diseases
In Situ Hybridization
Fluorescent Antibody Technique
Water

Keywords

  • cystic fibrosis

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Engelhardt, J. F., Smith, S. S., Allen, E., Yankaskas, J. R., Dawson, D. C., & Wilson, J. M. (1994). Coupled secretion of chloride and mucus in skin of Xenopus laevis: Possible role for CFTR. American Journal of Physiology - Cell Physiology, 267(2 36-2).

Coupled secretion of chloride and mucus in skin of Xenopus laevis : Possible role for CFTR. / Engelhardt, J. F.; Smith, S. S.; Allen, E.; Yankaskas, J. R.; Dawson, D. C.; Wilson, J. M.

In: American Journal of Physiology - Cell Physiology, Vol. 267, No. 2 36-2, 1994.

Research output: Contribution to journalArticle

Engelhardt, JF, Smith, SS, Allen, E, Yankaskas, JR, Dawson, DC & Wilson, JM 1994, 'Coupled secretion of chloride and mucus in skin of Xenopus laevis: Possible role for CFTR', American Journal of Physiology - Cell Physiology, vol. 267, no. 2 36-2.
Engelhardt, J. F. ; Smith, S. S. ; Allen, E. ; Yankaskas, J. R. ; Dawson, D. C. ; Wilson, J. M. / Coupled secretion of chloride and mucus in skin of Xenopus laevis : Possible role for CFTR. In: American Journal of Physiology - Cell Physiology. 1994 ; Vol. 267, No. 2 36-2.
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