Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences

Jennifer Y. Marciniak, Andrew C. Kummel, Sadik C. Esener, Michael J. Heller, Bradley T. Messmer

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplification reaction concurrent with RCA to achieve rapid signal development from the starting circular molecules. This isothermal reaction was found to be significantly faster than the comparable hyperbranching amplification method and could detect 100 circular copies in less than 1 h.

Original languageEnglish (US)
Pages (from-to)275-280
Number of pages6
JournalBioTechniques
Volume45
Issue number3
DOIs
StatePublished - Sep 1 2008

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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