Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences

Jennifer Y. Marciniak, Andrew C. Kummel, Sadik C. Esener, Michael J. Heller, Bradley T. Messmer

Research output: Contribution to journalArticle

12 Scopus citations


Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplification reaction concurrent with RCA to achieve rapid signal development from the starting circular molecules. This isothermal reaction was found to be significantly faster than the comparable hyperbranching amplification method and could detect 100 circular copies in less than 1 h.

Original languageEnglish (US)
Pages (from-to)275-280
Number of pages6
Issue number3
StatePublished - Sep 1 2008


ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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