Coupled gating between cardiac calcium release channels (ryanodine receptors)

Steven O. Marx, Jana Gaburjakova, Marta Gaburjakova, Charles Henrikson, Karol Ondrias, Andrew R. Marks

Research output: Contribution to journal › Article

305 Citations (Scopus)

Abstract

Excitation-contraction coupling in heart muscle requires the activation of Ca2+-release channels/type 2 ryanodine receptors (RyR2s) by Ca2+ influx. RyR2s are arranged on the sarcoplasmic reticular membrane in closely packed arrays such that their large cytoplasmic domains contact one another. We now show that multiple RyR2s can be isolated under conditions such that they remain physically coupled to one another. When these coupled channels are examined in planar lipid bilayers, multiple channels exhibit simultaneous gating, termed "coupled gating." Removal of the regulatory subunit, the FK506 binding protein (FKBP12.6), functionally but not physically uncouples multiple RyR2 channels. Coupled gating between RyR2 channels may be an important regulatory mechanism in excitation-contraction coupling as well as in other signaling pathways involving intracellular Ca2+ release.

Original languageEnglish (US)
Pages (from-to)1151-1158
Number of pages8
JournalCirculation Research
Volume88
Issue number11
StatePublished - Jun 8 2001
Externally publishedYes

Fingerprint

Ryanodine Receptor Calcium Release Channel
Excitation Contraction Coupling
Tacrolimus Binding Proteins
Lipid Bilayers
Myocardium
Membranes

Keywords

  • Ca channels
  • Coupled gating
  • Excitation-contraction coupling
  • FKBP12.6
  • Ryanodine receptors

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Marx, S. O., Gaburjakova, J., Gaburjakova, M., Henrikson, C., Ondrias, K., & Marks, A. R. (2001). Coupled gating between cardiac calcium release channels (ryanodine receptors). Circulation Research, 88(11), 1151-1158.

Coupled gating between cardiac calcium release channels (ryanodine receptors). / Marx, Steven O.; Gaburjakova, Jana; Gaburjakova, Marta; Henrikson, Charles; Ondrias, Karol; Marks, Andrew R.

In: Circulation Research, Vol. 88, No. 11, 08.06.2001, p. 1151-1158.

Research output: Contribution to journal › Article

Marx, SO, Gaburjakova, J, Gaburjakova, M, Henrikson, C, Ondrias, K & Marks, AR 2001, 'Coupled gating between cardiac calcium release channels (ryanodine receptors)', Circulation Research, vol. 88, no. 11, pp. 1151-1158.
Marx SO, Gaburjakova J, Gaburjakova M, Henrikson C, Ondrias K, Marks AR. Coupled gating between cardiac calcium release channels (ryanodine receptors). Circulation Research. 2001 Jun 8;88(11):1151-1158.
Marx, Steven O. ; Gaburjakova, Jana ; Gaburjakova, Marta ; Henrikson, Charles ; Ondrias, Karol ; Marks, Andrew R. / Coupled gating between cardiac calcium release channels (ryanodine receptors). In: Circulation Research. 2001 ; Vol. 88, No. 11. pp. 1151-1158.
@article{73945968d49c4b2ebc57ea555e222c63,
title = "Coupled gating between cardiac calcium release channels (ryanodine receptors)",
abstract = "Excitation-contraction coupling in heart muscle requires the activation of Ca2+-release channels/type 2 ryanodine receptors (RyR2s) by Ca2+ influx. RyR2s are arranged on the sarcoplasmic reticular membrane in closely packed arrays such that their large cytoplasmic domains contact one another. We now show that multiple RyR2s can be isolated under conditions such that they remain physically coupled to one another. When these coupled channels are examined in planar lipid bilayers, multiple channels exhibit simultaneous gating, termed {"}coupled gating.{"} Removal of the regulatory subunit, the FK506 binding protein (FKBP12.6), functionally but not physically uncouples multiple RyR2 channels. Coupled gating between RyR2 channels may be an important regulatory mechanism in excitation-contraction coupling as well as in other signaling pathways involving intracellular Ca2+ release.",
keywords = "Ca channels, Coupled gating, Excitation-contraction coupling, FKBP12.6, Ryanodine receptors",
author = "Marx, {Steven O.} and Jana Gaburjakova and Marta Gaburjakova and Charles Henrikson and Karol Ondrias and Marks, {Andrew R.}",
year = "2001",
month = "6",
day = "8",
language = "English (US)",
volume = "88",
pages = "1151--1158",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins",
number = "11",

}

TY - JOUR

T1 - Coupled gating between cardiac calcium release channels (ryanodine receptors)

AU - Marx, Steven O.

AU - Gaburjakova, Jana

AU - Gaburjakova, Marta

AU - Henrikson, Charles

AU - Ondrias, Karol

AU - Marks, Andrew R.

PY - 2001/6/8

Y1 - 2001/6/8

N2 - Excitation-contraction coupling in heart muscle requires the activation of Ca2+-release channels/type 2 ryanodine receptors (RyR2s) by Ca2+ influx. RyR2s are arranged on the sarcoplasmic reticular membrane in closely packed arrays such that their large cytoplasmic domains contact one another. We now show that multiple RyR2s can be isolated under conditions such that they remain physically coupled to one another. When these coupled channels are examined in planar lipid bilayers, multiple channels exhibit simultaneous gating, termed "coupled gating." Removal of the regulatory subunit, the FK506 binding protein (FKBP12.6), functionally but not physically uncouples multiple RyR2 channels. Coupled gating between RyR2 channels may be an important regulatory mechanism in excitation-contraction coupling as well as in other signaling pathways involving intracellular Ca2+ release.

AB - Excitation-contraction coupling in heart muscle requires the activation of Ca2+-release channels/type 2 ryanodine receptors (RyR2s) by Ca2+ influx. RyR2s are arranged on the sarcoplasmic reticular membrane in closely packed arrays such that their large cytoplasmic domains contact one another. We now show that multiple RyR2s can be isolated under conditions such that they remain physically coupled to one another. When these coupled channels are examined in planar lipid bilayers, multiple channels exhibit simultaneous gating, termed "coupled gating." Removal of the regulatory subunit, the FK506 binding protein (FKBP12.6), functionally but not physically uncouples multiple RyR2 channels. Coupled gating between RyR2 channels may be an important regulatory mechanism in excitation-contraction coupling as well as in other signaling pathways involving intracellular Ca2+ release.

KW - Ca channels

KW - Coupled gating

KW - Excitation-contraction coupling

KW - FKBP12.6

KW - Ryanodine receptors

UR - http://www.scopus.com/inward/record.url?scp=0035827726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035827726&partnerID=8YFLogxK

M3 - Article

C2 - 11397781

AN - SCOPUS:0035827726

VL - 88

SP - 1151

EP - 1158

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 11

ER -

Access to Document