Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack

Allen Volchuk, Mariella Ravazzola, Alain Perrelet, William S. Eng, Maurizio Di Liberto, Oleg Varlamov, Masayoshi Fukasawa, Thomas Engel, Thomas H. Söllner, James E. Rothman, Lelio Orci

    Research output: Contribution to journalArticle

    70 Scopus citations

    Abstract

    Genetic and biochemical evidence has established that a SNARE complex consisting of syntaxin 5 (Sed5)-mYkt6 (Ykt6)-GOS28 (Gos1)-GS15 (Sft1) is required for transport of proteins across the Golgi stack in animals (yeast). We have utilized quantitative immunogold labeling to establish the cis-trans distribution of the v-SNARE GS15 and the t-SNARE subunits GOS28 and syntaxin 5. Whereas the distribution of the t-SNARE is nearly even across the Golgi stack from the cis to the trans side, the v-SNARE GS15 is present in a gradient of increasing concentration toward the trans face of the stack. This contrasts with a second distinct SNARE complex, also required for intra-Golgi transport, consisting of syntaxin 5 (Sed5)-membrin (Bos1)-ERS24 (Sec22)-rBet1 (Bet1), whose v- (rBet1) and t-SNARE subunits (membrin and ERS24), progressively decrease in concentration toward the trans face. Transport within the stack therefore appears to utilize countercurrent gradients of two Golgi SNAREpins and may involve a mechanism akin to homotypic fusion.

    Original languageEnglish (US)
    Pages (from-to)1506-1518
    Number of pages13
    JournalMolecular biology of the cell
    Volume15
    Issue number4
    DOIs
    StatePublished - Apr 2004

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

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    Volchuk, A., Ravazzola, M., Perrelet, A., Eng, W. S., Di Liberto, M., Varlamov, O., Fukasawa, M., Engel, T., Söllner, T. H., Rothman, J. E., & Orci, L. (2004). Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack. Molecular biology of the cell, 15(4), 1506-1518. https://doi.org/10.1091/mbc.E03-08-0625