Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack

Allen Volchuk, Mariella Ravazzola, Alain Perrelet, William S. Eng, Maurizio Di Liberto, Oleg Varlamov, Masayoshi Fukasawa, Thomas Engel, Thomas H. Söllner, James E. Rothman, Lelio Orci

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Genetic and biochemical evidence has established that a SNARE complex consisting of syntaxin 5 (Sed5)-mYkt6 (Ykt6)-GOS28 (Gos1)-GS15 (Sft1) is required for transport of proteins across the Golgi stack in animals (yeast). We have utilized quantitative immunogold labeling to establish the cis-trans distribution of the v-SNARE GS15 and the t-SNARE subunits GOS28 and syntaxin 5. Whereas the distribution of the t-SNARE is nearly even across the Golgi stack from the cis to the trans side, the v-SNARE GS15 is present in a gradient of increasing concentration toward the trans face of the stack. This contrasts with a second distinct SNARE complex, also required for intra-Golgi transport, consisting of syntaxin 5 (Sed5)-membrin (Bos1)-ERS24 (Sec22)-rBet1 (Bet1), whose v- (rBet1) and t-SNARE subunits (membrin and ERS24), progressively decrease in concentration toward the trans face. Transport within the stack therefore appears to utilize countercurrent gradients of two Golgi SNAREpins and may involve a mechanism akin to homotypic fusion.

Original languageEnglish (US)
Pages (from-to)1506-1518
Number of pages13
JournalMolecular Biology of the Cell
Volume15
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

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Countercurrent Distribution
SNARE Proteins
Qa-SNARE Proteins
Molecular Biology
Carrier Proteins
Yeasts

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack. / Volchuk, Allen; Ravazzola, Mariella; Perrelet, Alain; Eng, William S.; Di Liberto, Maurizio; Varlamov, Oleg; Fukasawa, Masayoshi; Engel, Thomas; Söllner, Thomas H.; Rothman, James E.; Orci, Lelio.

In: Molecular Biology of the Cell, Vol. 15, No. 4, 04.2004, p. 1506-1518.

Research output: Contribution to journalArticle

Volchuk, A, Ravazzola, M, Perrelet, A, Eng, WS, Di Liberto, M, Varlamov, O, Fukasawa, M, Engel, T, Söllner, TH, Rothman, JE & Orci, L 2004, 'Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack', Molecular Biology of the Cell, vol. 15, no. 4, pp. 1506-1518. https://doi.org/10.1091/mbc.E03-08-0625
Volchuk, Allen ; Ravazzola, Mariella ; Perrelet, Alain ; Eng, William S. ; Di Liberto, Maurizio ; Varlamov, Oleg ; Fukasawa, Masayoshi ; Engel, Thomas ; Söllner, Thomas H. ; Rothman, James E. ; Orci, Lelio. / Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack. In: Molecular Biology of the Cell. 2004 ; Vol. 15, No. 4. pp. 1506-1518.
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