Counteractive balancing of transcriptome expression involving CodY and CovRS in Streptococcus pyogenes

Jens Kreth, Zhiyun Chen, Joseph Ferretti, Horst Malke

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Streptococcus pyogenes (group A streptococcus [GAS]) responds to environmental changes in a manner that results in an adaptive regulation of the transcriptome. The objective of the present study was to understand how two global transcriptional regulators, CodY and CovRS, coordinate the transcriptional network in S. pyogenes. Results from expression microarray data and quantitative reverse transcription-PCR (qRT-PCR) showed that the global regulator CodY controls the expression of about 250 genes, or about 17% of the genome of strain NZ131. Additionally, the codY gene was shown to be negatively autoregulated, with its protein binding directly to the promoter region with a CodY binding site. In further studies, the influence of codY, covRS, and codY-covRS mutations on gene expression was analyzed in growth phase-dependent conditions using C medium, reported to mimic nutritional abundance and famine conditions similar to those found during host GAS infection. Additional biological experiments of several virulence phenotypes, including pilin production, biofilm formation, and NAD glycohydrolase activity, demonstrated the role that both CodY and CovRS play in their regulation. Correlation analysis of the overall data revealed that, in exponentially growing cells, CodY and CovRS act in opposite directions, with CodY stimulating and CovRS repressing a substantial fraction of the core genome, including many virulence factors. This is the first report of counteractive balancing of transcriptome expression by global transcription regulators and provides important insight into how GAS modulates gene expression by integrating important extracellular and intracellular information.

Original languageEnglish (US)
Pages (from-to)4153-4165
Number of pages13
JournalJournal of Bacteriology
Volume193
Issue number16
DOIs
StatePublished - Aug 1 2011
Externally publishedYes

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Streptococcus pyogenes
Streptococcus
Transcriptome
NAD+ Nucleosidase
Fimbriae Proteins
Genome
Gene Expression
Gene Regulatory Networks
Virulence Factors
Biofilms
Starvation
Genetic Promoter Regions
Protein Binding
Genes
Reverse Transcription
Virulence
Binding Sites
Phenotype
Polymerase Chain Reaction
Mutation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Counteractive balancing of transcriptome expression involving CodY and CovRS in Streptococcus pyogenes. / Kreth, Jens; Chen, Zhiyun; Ferretti, Joseph; Malke, Horst.

In: Journal of Bacteriology, Vol. 193, No. 16, 01.08.2011, p. 4153-4165.

Research output: Contribution to journalArticle

Kreth, Jens ; Chen, Zhiyun ; Ferretti, Joseph ; Malke, Horst. / Counteractive balancing of transcriptome expression involving CodY and CovRS in Streptococcus pyogenes. In: Journal of Bacteriology. 2011 ; Vol. 193, No. 16. pp. 4153-4165.
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