Corticotropin-releasing hormone and pro-opiomelanocortin gene expression in female monkeys with differences in sensitivity to stress

Maria Luisa Centeno, Rachel L. Sanchez, Arubala Reddy, Judy L. Cameron, Cynthia Bethea

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Background/Aims: The expressions of corticotropin-releasing hormone (CRH) and pro-opiomelanocortin (POMC) were assessed in brain tissue collected from nonstressed female cynomolgus monkeys previously categorized as highly stress resilient (HSR), medium stress resilient (MSR), or stress sensitive (SS) with respect to stress-induced anovulation. Methods: In situ hybridization and quantitative image analysis was used to measure mRNAs coding for CRH in the hypothalamic paraventricular nucleus (PVN) and thalamic center median-subfascicular complex (CM-Sf). Then, CRH neurons in the PVN were immunostained and the area of immunostaining was measured. Also, CRH fibers were immunostained in the central nucleus of the amygdala and the area of immunostaining was obtained. Finally, POMC mRNA expression was characterized in the hypothalamic infundibular nucleus. The groups were compared with ANOVA and Student-Newman-Keul's (SNK) post hoc comparison. Results: CRH mRNA was significantly elevated in the caudal PVN in the MSR and SS animals compared to HSR animals (p <0.05, SNK). There was a significant increase in average and total CRH-positive area in the MSR and SS groups compared to the HSR group (p <0.05, SNK). There was also a significant increase in CRH volume in the MSR and SS groups compared to the HSR group (p <0.05, SNK). In the CM-Sf, the average CRH optical density was significantly higher in the MSR and SS groups than in the HSR group (p <0.05, SNK). In the central nucleus of the amygdala, the area of CRH fiber staining was significantly higher in the SS group than in the MSR or HSR groups (p <0.05, SNK). There was no difference between the groups in POMC mRNA expression in the mediobasal hypothalamus. Conclusion: Macaques that exhibit immediate suppression of reproductive function upon stress are considered stress sensitive. These animals have elevated CRH in the hypothalamus and limbic structures, which may play a role in suppressing the hypothalamic-gonadal axis upon stress initiation.

    Original languageEnglish (US)
    Pages (from-to)277-288
    Number of pages12
    JournalNeuroendocrinology
    Volume86
    Issue number4
    DOIs
    StatePublished - Nov 2007

    Fingerprint

    Pro-Opiomelanocortin
    Corticotropin-Releasing Hormone
    Haplorhini
    Gene Expression
    Students
    Paraventricular Hypothalamic Nucleus
    Messenger RNA
    Hypothalamus
    Midline Thalamic Nuclei
    Anovulation
    Arcuate Nucleus of Hypothalamus
    Macaca fascicularis
    Macaca
    In Situ Hybridization
    Analysis of Variance
    Staining and Labeling
    Neurons

    Keywords

    • Amygdala
    • Beta-endorphin
    • Corticotropin-releasing hormone
    • Cynomolgus macaque
    • Paraventricular nucleus
    • Pro-opiomelanocortin
    • Reproduction
    • Stress
    • Thalamus

    ASJC Scopus subject areas

    • Endocrinology
    • Neuroscience(all)

    Cite this

    Corticotropin-releasing hormone and pro-opiomelanocortin gene expression in female monkeys with differences in sensitivity to stress. / Centeno, Maria Luisa; Sanchez, Rachel L.; Reddy, Arubala; Cameron, Judy L.; Bethea, Cynthia.

    In: Neuroendocrinology, Vol. 86, No. 4, 11.2007, p. 277-288.

    Research output: Contribution to journalArticle

    Centeno, Maria Luisa ; Sanchez, Rachel L. ; Reddy, Arubala ; Cameron, Judy L. ; Bethea, Cynthia. / Corticotropin-releasing hormone and pro-opiomelanocortin gene expression in female monkeys with differences in sensitivity to stress. In: Neuroendocrinology. 2007 ; Vol. 86, No. 4. pp. 277-288.
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    abstract = "Background/Aims: The expressions of corticotropin-releasing hormone (CRH) and pro-opiomelanocortin (POMC) were assessed in brain tissue collected from nonstressed female cynomolgus monkeys previously categorized as highly stress resilient (HSR), medium stress resilient (MSR), or stress sensitive (SS) with respect to stress-induced anovulation. Methods: In situ hybridization and quantitative image analysis was used to measure mRNAs coding for CRH in the hypothalamic paraventricular nucleus (PVN) and thalamic center median-subfascicular complex (CM-Sf). Then, CRH neurons in the PVN were immunostained and the area of immunostaining was measured. Also, CRH fibers were immunostained in the central nucleus of the amygdala and the area of immunostaining was obtained. Finally, POMC mRNA expression was characterized in the hypothalamic infundibular nucleus. The groups were compared with ANOVA and Student-Newman-Keul's (SNK) post hoc comparison. Results: CRH mRNA was significantly elevated in the caudal PVN in the MSR and SS animals compared to HSR animals (p <0.05, SNK). There was a significant increase in average and total CRH-positive area in the MSR and SS groups compared to the HSR group (p <0.05, SNK). There was also a significant increase in CRH volume in the MSR and SS groups compared to the HSR group (p <0.05, SNK). In the CM-Sf, the average CRH optical density was significantly higher in the MSR and SS groups than in the HSR group (p <0.05, SNK). In the central nucleus of the amygdala, the area of CRH fiber staining was significantly higher in the SS group than in the MSR or HSR groups (p <0.05, SNK). There was no difference between the groups in POMC mRNA expression in the mediobasal hypothalamus. Conclusion: Macaques that exhibit immediate suppression of reproductive function upon stress are considered stress sensitive. These animals have elevated CRH in the hypothalamus and limbic structures, which may play a role in suppressing the hypothalamic-gonadal axis upon stress initiation.",
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    AU - Centeno, Maria Luisa

    AU - Sanchez, Rachel L.

    AU - Reddy, Arubala

    AU - Cameron, Judy L.

    AU - Bethea, Cynthia

    PY - 2007/11

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    N2 - Background/Aims: The expressions of corticotropin-releasing hormone (CRH) and pro-opiomelanocortin (POMC) were assessed in brain tissue collected from nonstressed female cynomolgus monkeys previously categorized as highly stress resilient (HSR), medium stress resilient (MSR), or stress sensitive (SS) with respect to stress-induced anovulation. Methods: In situ hybridization and quantitative image analysis was used to measure mRNAs coding for CRH in the hypothalamic paraventricular nucleus (PVN) and thalamic center median-subfascicular complex (CM-Sf). Then, CRH neurons in the PVN were immunostained and the area of immunostaining was measured. Also, CRH fibers were immunostained in the central nucleus of the amygdala and the area of immunostaining was obtained. Finally, POMC mRNA expression was characterized in the hypothalamic infundibular nucleus. The groups were compared with ANOVA and Student-Newman-Keul's (SNK) post hoc comparison. Results: CRH mRNA was significantly elevated in the caudal PVN in the MSR and SS animals compared to HSR animals (p <0.05, SNK). There was a significant increase in average and total CRH-positive area in the MSR and SS groups compared to the HSR group (p <0.05, SNK). There was also a significant increase in CRH volume in the MSR and SS groups compared to the HSR group (p <0.05, SNK). In the CM-Sf, the average CRH optical density was significantly higher in the MSR and SS groups than in the HSR group (p <0.05, SNK). In the central nucleus of the amygdala, the area of CRH fiber staining was significantly higher in the SS group than in the MSR or HSR groups (p <0.05, SNK). There was no difference between the groups in POMC mRNA expression in the mediobasal hypothalamus. Conclusion: Macaques that exhibit immediate suppression of reproductive function upon stress are considered stress sensitive. These animals have elevated CRH in the hypothalamus and limbic structures, which may play a role in suppressing the hypothalamic-gonadal axis upon stress initiation.

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    KW - Pro-opiomelanocortin

    KW - Reproduction

    KW - Stress

    KW - Thalamus

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