Corticotropin/β-lipotropin biosynthesis, processing, and release in Nelson's syndrome

R. Allen, Eric Orwoll, H. Kammer, John Kendall

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Abstract

Biosynthesis and processing of ACTH/β-lipotropin was studied in Nelson's syndrome pituitary tumor tissue grown in monolayer culture. Radiolabeled peptides were immunoprecipitated and fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE). Important findings include: (1) a virtual absence of 13K ACTH or 3.5K β-endorphin production; (2) evidence indicating the presence of a 24-26K ACTH and β-LPH containing intermediate (which implies a different order of processing from that reported in the mouse); (3) an extremely rapid rate of turnover and release of ACTH and β-lipotropin (β-LPH) similar to that of the mouse AtT20/D16v pituitary tumors. The latter finding is consistent with an intrinsic pituitary cell defect in the pathogenesis of this disorder.

Original languageEnglish (US)
Pages (from-to)887-889
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume53
Issue number4
Publication statusPublished - 1981

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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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