Corticosterone-induced rapid phosphorylation of p38 and JNK mitogen-activated protein kinases in PC12 cells

Xiaoyu Li; Jian Qiu; Jianwen Wang; Yongping Zhong; Jianqin Zhu; Yizhang Chen

doi:10.1016/S0014-5793(01)02254-2

Corticosterone-induced rapid phosphorylation of p38 and JNK mitogen-activated protein kinases in PC12 cells

Xiaoyu Li, Jian Qiu, Jianwen Wang, Yongping Zhong, Jianqin Zhu, Yizhang Chen

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The present study showed that corticosterone (B) could induce a rapid activation of p38 and c-Jun NH₂-terminal protein kinase (JNK) in PC12 cells. The dose-response and time-response curves were bell-shaped with maximal activation at 10^-9 M and at 15 min. RU38486 had no effect, and bovine serum albumin-coupled B could induce the activation. Genistein failed to block the phosphorylation, suggesting the pathway was not involved in tyrosine kinase activity. Phorbol 12-myristate 13-acetate could mimic, while Gö6976 could abolish the actions. These results demonstrated that B might act via a putative membrane receptor to activate p38 and JNK rapidly through a protein kinase C-dependent pathway.

Original language	English (US)
Pages (from-to)	210-214
Number of pages	5
Journal	FEBS Letters
Volume	492
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(01)02254-2
State	Published - Mar 16 2001
Externally published	Yes

Keywords

Corticosterone
Non-genomic
PC12 cell
Protein kinase C
c-Jun NH-terminal protein kinase
p38

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)02254-2

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title = "Corticosterone-induced rapid phosphorylation of p38 and JNK mitogen-activated protein kinases in PC12 cells",

abstract = "The present study showed that corticosterone (B) could induce a rapid activation of p38 and c-Jun NH2-terminal protein kinase (JNK) in PC12 cells. The dose-response and time-response curves were bell-shaped with maximal activation at 10-9 M and at 15 min. RU38486 had no effect, and bovine serum albumin-coupled B could induce the activation. Genistein failed to block the phosphorylation, suggesting the pathway was not involved in tyrosine kinase activity. Phorbol 12-myristate 13-acetate could mimic, while G{\"o}6976 could abolish the actions. These results demonstrated that B might act via a putative membrane receptor to activate p38 and JNK rapidly through a protein kinase C-dependent pathway.",

keywords = "Corticosterone, Non-genomic, PC12 cell, Protein kinase C, c-Jun NH-terminal protein kinase, p38",

author = "Xiaoyu Li and Jian Qiu and Jianwen Wang and Yongping Zhong and Jianqin Zhu and Yizhang Chen",

note = "Funding Information: This work was supported by the National Basic Research Program of China (G1999054000), and by National Natural Science Foundation (NSF) grants (No. 39840019 and No. 39330100). The authors thank Xiaolu Tang (Department of Physiology, Second Military Medical University) for her technical assistance. ",

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doi = "10.1016/S0014-5793(01)02254-2",

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T1 - Corticosterone-induced rapid phosphorylation of p38 and JNK mitogen-activated protein kinases in PC12 cells

AU - Li, Xiaoyu

AU - Qiu, Jian

AU - Wang, Jianwen

AU - Zhong, Yongping

AU - Zhu, Jianqin

AU - Chen, Yizhang

N1 - Funding Information: This work was supported by the National Basic Research Program of China (G1999054000), and by National Natural Science Foundation (NSF) grants (No. 39840019 and No. 39330100). The authors thank Xiaolu Tang (Department of Physiology, Second Military Medical University) for her technical assistance.

PY - 2001/3/16

Y1 - 2001/3/16

N2 - The present study showed that corticosterone (B) could induce a rapid activation of p38 and c-Jun NH2-terminal protein kinase (JNK) in PC12 cells. The dose-response and time-response curves were bell-shaped with maximal activation at 10-9 M and at 15 min. RU38486 had no effect, and bovine serum albumin-coupled B could induce the activation. Genistein failed to block the phosphorylation, suggesting the pathway was not involved in tyrosine kinase activity. Phorbol 12-myristate 13-acetate could mimic, while Gö6976 could abolish the actions. These results demonstrated that B might act via a putative membrane receptor to activate p38 and JNK rapidly through a protein kinase C-dependent pathway.

AB - The present study showed that corticosterone (B) could induce a rapid activation of p38 and c-Jun NH2-terminal protein kinase (JNK) in PC12 cells. The dose-response and time-response curves were bell-shaped with maximal activation at 10-9 M and at 15 min. RU38486 had no effect, and bovine serum albumin-coupled B could induce the activation. Genistein failed to block the phosphorylation, suggesting the pathway was not involved in tyrosine kinase activity. Phorbol 12-myristate 13-acetate could mimic, while Gö6976 could abolish the actions. These results demonstrated that B might act via a putative membrane receptor to activate p38 and JNK rapidly through a protein kinase C-dependent pathway.

KW - Corticosterone

KW - Non-genomic

KW - PC12 cell

KW - Protein kinase C

KW - c-Jun NH-terminal protein kinase

KW - p38

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JO - FEBS Letters

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Corticosterone-induced rapid phosphorylation of p38 and JNK mitogen-activated protein kinases in PC12 cells

Abstract

Keywords

ASJC Scopus subject areas

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