Corticosterone activates Erk1/2 mitogen-activated protein kinase in primary hippocampal cells through rapid nongenomic mechanism

Aiqun Qi, Jian Qiu, Lin Xiao, Yizhang Chen

Research output: Contribution to journalArticle

Abstract

Nongenomic effects of glucocorticoids (GC) in various cell types have been well documented, but it still remains unknown whether the mechanism also works in hippocampus which is a crucial target of glucocorticoids in neural system during physiological and/or pathophysiological processes. We present here that corticosterone (B) could rapidly activate Erk1/2 mitogen-activated protein kinase (MAPK) in primarily cultured hippocampal cells within minutes, with a bell-shaped time dependent curve which peaked at 15min and then went down to normal level in 30 min. This activation was blocked by protein kinase C (PKC) inhibitor (Go6976), G protein inhibitor (GDPβs), and MEK (MAPK/extracellular signal-regulated kinase kinase) inhibitor (PD98059), but not by protein kinase A (PKA) inbibitor (H89), tyrosine kinase inhibitor (genistein), and glucocorticoid receptor (GR) antagonist (RU38486). Thus, the rapid activation of Erk1/2 MAPK in primary hippocampal cells induced by B was likely mediated by a G protein coupled receptor (GPCR) pathway with involvement of PKC, which belonged to the nongenomic rather than genomic mechanism of GC's effects.

Original languageEnglish (US)
Pages (from-to)325-330
Number of pages6
JournalProgress in Natural Science
Volume15
Issue number4
StatePublished - Apr 2005
Externally publishedYes

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Mitogen-Activated Protein Kinase 1
Corticosterone
Protein Kinase C
Glucocorticoids
Chemical activation
Proteins
Genistein
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Glucocorticoid Receptors
G-Protein-Coupled Receptors
Cyclic AMP-Dependent Protein Kinases
Mitogen-Activated Protein Kinases
GTP-Binding Proteins
Protein-Tyrosine Kinases
Phosphotransferases
Go 6976
N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

Keywords

  • Glucocorticoid
  • MAPK
  • Nongenomic mechanism.
  • Rapid effects

ASJC Scopus subject areas

  • General

Cite this

Corticosterone activates Erk1/2 mitogen-activated protein kinase in primary hippocampal cells through rapid nongenomic mechanism. / Qi, Aiqun; Qiu, Jian; Xiao, Lin; Chen, Yizhang.

In: Progress in Natural Science, Vol. 15, No. 4, 04.2005, p. 325-330.

Research output: Contribution to journalArticle

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