Correspondence between plasma mevalonic acid levels and deuterium uptake in measuring human cholesterol synthesis

P. J.H. JONES, A. S. PAPPU, D. R. ILLINGWORTH, C. A. LEITCH

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Abstract. To assess the validity of two techniques capable of identifying immediate changes in human cholesterol production, plasma mevalonic acid levels and the rate of uptake of deuterium into plasma free cholesterol were compared in 5 healthy individuals over 48 h. The free‐living subjects self‐selected three meals per day prior to and during study. At t = 0, deuterium oxide was administered orally. Blood samples were collected before and every 4 h after dosing. Total cholesterol and mevalonic acid levels were determined in plasma at each timepoint. Deuterium enrichment changes in plasma free cholesterol, relative to plasma water content, were used to calculate free cholesterol fractional synthetic rates (FSR) at each timepoint. Total plasma cholesterol levels remained constant, whereas significant circadian rhythmicity was observed in both plasma mevalonic acid and deuterium uptake methods, with nadir and peak formation rates indicated at 14.00 to 16.00 h and about midnight, respectively. It is suggested that plasma mevalonic acid levels and free cholesterol deuterium uptake rate techniques are both suitable techniques for short‐term measurement of human cholesterol synthesis.

Original languageEnglish (US)
Pages (from-to)609-613
Number of pages5
JournalEuropean Journal of Clinical Investigation
Volume22
Issue number9
DOIs
StatePublished - Sep 1992

Keywords

  • Cholesterol
  • circadian periodicity
  • deuterium
  • mevalonic acid
  • synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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