Corneal stroma microfibrils

Samuel D. Hanlon, Ali R. Behzad, Lynn Sakai, Alan R. Burns

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

Elastic tissue was first described well over a hundred years ago and has since been identified in nearly every part of the body. In this review, we examine elastic tissue in the corneal stroma with some mention of other ocular structures which have been more thoroughly described in the past. True elastic fibers consist of an elastin core surrounded by fibrillin microfibrils. However, the presence of elastin fibers is not a requirement and some elastic tissue is comprised of non-elastin-containing bundles of microfibrils. Fibers containing a higher relative amount of elastin are associated with greater elasticity and those without elastin, with structural support. Recently it has been shown that the microfibrils, not only serve mechanical roles, but are also involved in cell signaling through force transduction and the release of TGF-β. A well characterized example of elastin-free microfibril bundles (EFMBs) is found in the ciliary zonules which suspend the crystalline lens in the eye. Through contraction of the ciliary muscle they exert enough force to reshape the lens and thereby change its focal point. It is believed that the molecules comprising these fibers do not turn-over and yet retain their tensile strength for the life of the animal. The mechanical properties of the cornea (strength, elasticity, resiliency) would suggest that EFMBs are present there as well. However, many authors have reported that, although present during embryonic and early postnatal development, EFMBs are generally not present in adults. Serial-block-face imaging with a scanning electron microscope enabled 3D reconstruction of elements in murine corneas. Among these elements were found fibers that formed an extensive network throughout the cornea. In single sections these fibers appeared as electron dense patches. Transmission electron microscopy provided additional detail of these patches and showed them to be composed of fibrils (~10nm diameter). Immunogold evidence clearly identified these fibrils as fibrillin EFMBs and EFMBs were also observed with TEM (without immunogold) in adult mammals of several species. Evidence of the presence of EFMBs in adult corneas will hopefully pique an interest in further studies that will ultimately improve our understanding of the cornea's biomechanical properties and its capacity to repair.

Original languageEnglish (US)
Pages (from-to)198-207
Number of pages10
JournalExperimental Eye Research
Volume132
DOIs
StatePublished - Mar 1 2015

Fingerprint

Microfibrils
Corneal Stroma
Elastin
Elastic Tissue
Cornea
Elasticity
Electrons
Crystalline Lens
Tensile Strength
Muscle Contraction
Transmission Electron Microscopy
Human Body
Lenses
Mammals

Keywords

  • Cornea
  • Elastic tissue
  • Fibrillin
  • Microfibrils
  • Oxytalan

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Hanlon, S. D., Behzad, A. R., Sakai, L., & Burns, A. R. (2015). Corneal stroma microfibrils. Experimental Eye Research, 132, 198-207. https://doi.org/10.1016/j.exer.2015.01.014

Corneal stroma microfibrils. / Hanlon, Samuel D.; Behzad, Ali R.; Sakai, Lynn; Burns, Alan R.

In: Experimental Eye Research, Vol. 132, 01.03.2015, p. 198-207.

Research output: Contribution to journalReview article

Hanlon, SD, Behzad, AR, Sakai, L & Burns, AR 2015, 'Corneal stroma microfibrils', Experimental Eye Research, vol. 132, pp. 198-207. https://doi.org/10.1016/j.exer.2015.01.014
Hanlon, Samuel D. ; Behzad, Ali R. ; Sakai, Lynn ; Burns, Alan R. / Corneal stroma microfibrils. In: Experimental Eye Research. 2015 ; Vol. 132. pp. 198-207.
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