Corneal imaging with blue-light optical coherence microscopy

Shanjida Khan, Kai Neuhaus, Omkar Thaware, Shuibin Ni, Myeong Jin Ju, Travis Redd, David Huang, Yifan Jian

Research output: Contribution to journalArticlepeer-review

Abstract

Corneal imaging is important for the diagnostic and therapeutic evaluation of many eye diseases. Optical coherence tomography (OCT) is extensively used in ocular imaging due to its non-invasive and high-resolution volumetric imaging characteristics. Optical coherence microscopy (OCM) is a technical variation of OCT that can image the cornea with cellular resolution. Here, we demonstrate a blue-light OCM as a low-cost and easily reproducible system to visualize corneal cellular structures such as epithelial cells, endothelial cells, keratocytes, and collagen bundles within stromal lamellae. Our blue-light OCM system achieved an axial resolution of 12 µm in tissue over a 1.2 mm imaging depth, and a lateral resolution of 1.6 µm over a field of view of 750 µm × 750 µm.

Original languageEnglish (US)
Pages (from-to)5004-5014
Number of pages11
JournalBiomedical Optics Express
Volume13
Issue number9
DOIs
StatePublished - Sep 1 2022

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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