Coordinate expression of NGF and α-smooth muscle actin mRNA and protein in cutaneous wound tissue of developing and adult rats

Wohaib Hasan, Renjie Zhang, Manxi Liu, J. Donald Warn, Peter G. Smith

    Research output: Contribution to journalArticlepeer-review

    64 Scopus citations

    Abstract

    Nerve growth factor (NGF) is synthesized in cutaneous wound tissue, and its higher levels in the neonate may contribute to more efficient wound healing. We used in situ hybridization and immunohistochemistry to define NGF mRNA and protein expression in intact skin and following excision wounding in neonatal and adult rats. To determine whether NGF is associated with wound contractile fibroblasts (myofibroblasts), we also examined expression of α-smooth muscle actin (α-SMA) mRNA and protein, established markers for these cells. In intact skin, NGF mRNA and protein were present in vascular and arrector pili smooth muscle, hair follicle sheath cells, keratinocytes, and hypodermal fibroblasts. Neonatal adipocytes and Schwann cells also expressed NGF mRNA and protein, while adult adipocytes and Schwann cells displayed only NGF-ir. Following wounding, NGF mRNA expression was exuberant in these cell types, and increased similarly at both ages and appeared de novo in skeletal muscle cells. Additionally, both NGF mRNA and protein were present in macrophages and myofibroblasts, and expression in myofibroblasts was significantly greater in neonates. Wound myofibroblasts also expressed α-SMA. Surprisingly, after wounding α-SMA mRNA and protein were present in essentially all cells in which NGF mRNA was detected. We conclude that NGF expression is enhanced in many cell types after wounding, but greater NGF synthesis in neonates appears to be due to a more robust myofibroblast response. In addition, cell types which demonstrated NGF mRNA also expressed α-SMA, and staining for both markers increased following wounding, suggesting synthesis of both proteins is regulated in a coordinated fashion.

    Original languageEnglish (US)
    Pages (from-to)97-109
    Number of pages13
    JournalCell and tissue research
    Volume300
    Issue number1
    DOIs
    StatePublished - Apr 28 2000

    Keywords

    • Development
    • In situ hybridization
    • Macrophages
    • Myofibroblasts
    • Nerve growth factor
    • Rat (Sprague-Dawley)
    • Skin
    • Wound

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine
    • Histology
    • Cell Biology

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