Coordinate estrogen induction of vitellogenin and a small serum protein mRNA in Xenopus laevis liver

Marshall A. Hayward, Michelle C. Barton, David J. Shapiro

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We have used plus-minus hybridization to identify Xenopus liver cDNA clones of mRNAs whose levels are regulated by estrogen. One clone identified in this way was shown to be a nearly full-length cDNA clone of the mRNA coding for a small 22 000 dalton estrogen-inducible serum protein (EISP). Quantitation of EISP mRNA levels by in vitro translation and by hybridization to the cloned DNA demonstrated a 7-12-fold estrogen induction of EISP mRNA, both in vivo and in primary Xenopus liver cultures. The kinetics of induction of EISP mRNA closely parallel those of the mRNA coding for the abundant estrogen-inducible serum protein, vitellogenin. In contrast, the massive, and toxic, estrogen-mediated accumulation of vitellogenin in serum of male Xenopus laevis is accompanied by a sharp decline in the levels of albumin mRNA and in the levels of the mRNAs coding for several other serum proteins.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume39
Issue number2
DOIs
StatePublished - Feb 1985
Externally publishedYes

Keywords

  • gene expression

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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