Controlled vascular corrosion casting of the rabbit eye

W. H. Fahrenbach, D. R. Bacon, John Morrison, E. M. Van Buskirk

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have refined the technique of vascular corrosion casting with methacrylate to permit the reproduction of physiological states of vascular tone and to produce sturdy castings of ocular microvasculature. The method entails careful maintenance of homeostasis up to the moment of plastic perfusion, avoidance of vascular rinsing or fixation with attendant anoxia, reduction of the viscosity of the casting resin without impairing the properties of the resultant polymer, addition of a cross-linking agent to increase the strength of the plastic, and injection at physiological temperature and pressure. This casting regimen reproduces the normal anatomical conditions of blood vessels and can be used to demonstrate altered conditions of vascular tone. In all instances, the second, untouched eye serves as a control for unilateral manipulations. Special problems of replicating the ocular vasculature are related to the intraocular pressure, which opposes the vascular perfusion pressure and constitutes an impediment to perfusion.

Original languageEnglish (US)
Pages (from-to)15-26
Number of pages12
JournalJournal of Electron Microscopy Technique
Volume10
Issue number1
StatePublished - 1988

Fingerprint

Corrosion Casting
rabbits
Blood Vessels
corrosion
Casting
Corrosion
Rabbits
plastics
intraocular pressure
anoxia
Plastics
homeostasis
Perfusion
avoidance
blood vessels
Blood vessels
resins
maintenance
manipulators
Resins

ASJC Scopus subject areas

  • Anatomy

Cite this

Fahrenbach, W. H., Bacon, D. R., Morrison, J., & Van Buskirk, E. M. (1988). Controlled vascular corrosion casting of the rabbit eye. Journal of Electron Microscopy Technique, 10(1), 15-26.

Controlled vascular corrosion casting of the rabbit eye. / Fahrenbach, W. H.; Bacon, D. R.; Morrison, John; Van Buskirk, E. M.

In: Journal of Electron Microscopy Technique, Vol. 10, No. 1, 1988, p. 15-26.

Research output: Contribution to journalArticle

Fahrenbach, WH, Bacon, DR, Morrison, J & Van Buskirk, EM 1988, 'Controlled vascular corrosion casting of the rabbit eye', Journal of Electron Microscopy Technique, vol. 10, no. 1, pp. 15-26.
Fahrenbach, W. H. ; Bacon, D. R. ; Morrison, John ; Van Buskirk, E. M. / Controlled vascular corrosion casting of the rabbit eye. In: Journal of Electron Microscopy Technique. 1988 ; Vol. 10, No. 1. pp. 15-26.
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